Evidence for preferential T cell receptor Vβ gene usage and T cell clonal expansion in the synovium of BB rats with early-onset collagen- induced arthritis

Xiu J. Ye, Jacob Aelion, Robert O. Endres, Andrew Kang, Michael A. Cremer

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Type II collagen (CII) is a potent arthritogen in the BB rat. To determine whether a restricted group of T cells is involved in the pathogenesis of collagen-induced arthritis, lymphocytes from synovium, peripheral blood, and lymph nodes of arthritic rats were studied for T cell receptor (TCR) Vβ gene usage using polymerase chain reaction (PCR). Oligoclonal TCR Vβ usage was found only in synovium recovered day 2 post- arthritis onset, but not day 7; lymph node and peripheral blood T cells showed diverse TCR usage at both times. To determine whether T cell local clonal expansion occurred in synovium at day 2 of arthritis, cDNA for four TCR β families was sequenced through VDJ regions. Strong selective expansion of TCR Vβ 8.2, 4, and 17 was noted. Importantly, the dominant clonotype of Vβ 8.2 was identical to that of a lymph node-derived T hybridoma specific for the immunodominant epitope in CII(181-210). Cells from synovium (day 2 postonset) analyzed by flow cytometry also showed Vβ 8.2+ cell enrichment. These observations, plus finding that T cells from inflamed synovium respond to CII(181-201) in vitro, suggest the local recruitment and clonal expansion of some T cells families, possibly driven by autologous CII released during cartilage degradation.

Original languageEnglish (US)
Pages (from-to)81-89
Number of pages9
JournalCellular Immunology
Volume183
Issue number2
DOIs
StatePublished - Feb 1 1998

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Inbred BB Rats
T-Cell Receptor Genes
Experimental Arthritis
Synovial Membrane
T-Cell Antigen Receptor
T-Lymphocytes
Arthritis
Lymph Nodes
Immunodominant Epitopes
Collagen Type II
Hybridomas
Cartilage
Blood Cells
Flow Cytometry
Complementary DNA
Lymphocytes
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Evidence for preferential T cell receptor Vβ gene usage and T cell clonal expansion in the synovium of BB rats with early-onset collagen- induced arthritis. / Ye, Xiu J.; Aelion, Jacob; Endres, Robert O.; Kang, Andrew; Cremer, Michael A.

In: Cellular Immunology, Vol. 183, No. 2, 01.02.1998, p. 81-89.

Research output: Contribution to journalArticle

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