Evidence for the synthesis of tubulin on membrane-bound and free ribosomes from rat forebrain

Erik R. Floor, Jeffrey M. Gilbert, Thaddeus Nowak

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Evidence is presented that tubulin is synthesized in cell-free systems derived from rat forebrain containing either membrane-bound or free ribosomes. Both systems were supplemented with saturating amounts of messenger RNA (from either membrane-bound or free ribosomes) and soluble enzyme and initiation factors from whole forebrain. Synthesized products were characterized by two biochemical purification methods for brain tubulin isolation. These methods of tubulin analysis indicated that both cell free systems synthesized the same relative amounts of tubulin.

Original languageEnglish (US)
Pages (from-to)285-296
Number of pages12
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume442
Issue number3
DOIs
StatePublished - Sep 6 1976

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Tubulin
Prosencephalon
Ribosomes
Membranes
Cell-Free System
Peptide Initiation Factors
Messenger RNA
Brain
Enzymes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Evidence for the synthesis of tubulin on membrane-bound and free ribosomes from rat forebrain. / Floor, Erik R.; Gilbert, Jeffrey M.; Nowak, Thaddeus.

In: BBA Section Nucleic Acids And Protein Synthesis, Vol. 442, No. 3, 06.09.1976, p. 285-296.

Research output: Contribution to journalArticle

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