Exercise training enhances white adipose tissue metabolism in rats selectively bred for low- or high-endurance running capacity

Erin Stephenson, Sarah J. Lessard, Donato A. Rivas, Matthew J. Watt, Ben B. Yaspelkis, Lauren G. Koch, Steven L. Britton, John A. Hawley

Research output: Contribution to journalArticle

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Abstract

Impaired visceral white adipose tissue (WAT) metabolism has been implicated in the pathogenesis of several lifestyle-related disease states, with diminished expression of several WAT mitochondrial genes reported in both insulin-resistant humans and rodents. We have used rat models selectively bred for low- (LCR) or high-intrinsic running capacity (HCR) that present simultaneously with divergent metabolic phenotypes to test the hypothesis that oxidative enzyme expression is reduced in epididymal WAT from LCR animals. Based on this assumption, we further hypothesized that short-term exercise training (6 wk of treadmill running) would ameliorate this deficit. Approximately 22-wk-old rats (generation 22) were studied. In untrained rats, the abundance of mitochondrial respiratory complexes I-V, citrate synthase (CS), and PGC-1 was similar for both phenotypes, although CS activity was greater than 50% in HCR (P = 0.09). Exercise training increased CS activity in both phenotypes but did not alter mitochondrial protein content. Training increased the expression and phosphorylation of proteins with roles in β-adrenergic signaling, including β3-adrenergic receptor (16% increase in LCR; P < 0.05), NOR1 (24% decrease in LCR, 21% decrease in HCR; P < 0.05), phospho-ATGL (25% increase in HCR; P < 0.05), perilipin (25% increase in HCR; P < 0.05), CGI-58 (15% increase in LCR; P < 0.05), and GLUT4 (16% increase in HCR; P < 0.0001). A training effect was also observed for phospho-p38 MAPK (12% decrease in LCR, 20% decrease in HCR; P < 0.05) and phospho-JNK (29% increase in LCR, 20% increase in HCR; P < 0.05). We conclude that in the LCR-HCR model system, mitochondrial protein expression in WAT is not affected by intrinsic running capacity or exercise training. However, training does induce alterations in the activity and expression of several proteins that are essential to the intracellular regulation of WAT metabolism.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume305
Issue number3
DOIs
StatePublished - Aug 1 2013

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White Adipose Tissue
Running
Citrate (si)-Synthase
Exercise
Mitochondrial Proteins
Phenotype
Electron Transport Complex I
Mitochondrial Genes
Intra-Abdominal Fat
p38 Mitogen-Activated Protein Kinases
Adrenergic Agents
Adrenergic Receptors
Life Style
Rodentia
Proteins
Phosphorylation
Insulin
Enzymes

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Exercise training enhances white adipose tissue metabolism in rats selectively bred for low- or high-endurance running capacity. / Stephenson, Erin; Lessard, Sarah J.; Rivas, Donato A.; Watt, Matthew J.; Yaspelkis, Ben B.; Koch, Lauren G.; Britton, Steven L.; Hawley, John A.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 305, No. 3, 01.08.2013.

Research output: Contribution to journalArticle

Stephenson, Erin ; Lessard, Sarah J. ; Rivas, Donato A. ; Watt, Matthew J. ; Yaspelkis, Ben B. ; Koch, Lauren G. ; Britton, Steven L. ; Hawley, John A. / Exercise training enhances white adipose tissue metabolism in rats selectively bred for low- or high-endurance running capacity. In: American Journal of Physiology - Endocrinology and Metabolism. 2013 ; Vol. 305, No. 3.
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