Exploring the involvement of Tac2 in the mouse hippocampal stress response through gene networking

Mike Hook, Fuyi Xu, Elena Terenina, Wenyuan Zhao, Athena Davenport, Pierre Mormede, Byron Jones, Megan Mulligan, Lu Lu

Research output: Contribution to journalArticle

Abstract

Tachykinin 2 (Tac2) is expressed in a number of areas throughout the brain, including the hippocampus. However, knowledge about its function has been only well explored in the hypothalamus in the context of reproductive health. In this study, we identified and validated increased hippocampal Tac2 mRNA expression in response to chronic mild stress in mice. Expression quantitative trait locus (eQTL) analysis showed Tac2 is cis-regulated in the hippocampus. Using a systems genetics approach, we constructed a Tac2 co-expression network to better understand the relationship between Tac2 and the hippocampal stress response. Our network identified 69 total genes associated with Tac2, several of which encode major neuropeptides involved in hippocampal stress signaling as well as critical genes for producing neural plasticity, indicating that Tac2 is involved in these processes. Pathway analysis for the member of Tac2 gene network revealed a strong connection between Tac2 and neuroactive ligand-receptor interaction, calcium signaling pathway, as well as cardiac muscle contraction. In addition, we also identified 46 stress-related phenotypes, specifically fear conditioning response, that were significantly correlated with Tac2 expression. Our results provide evidence for Tac2 as a strong candidate gene who likely plays a role in hippocampal stress processing and neural plasticity.

Original languageEnglish (US)
Pages (from-to)176-185
Number of pages10
JournalGene
Volume696
DOIs
StatePublished - May 15 2019

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Tachykinins
Genes
Neuronal Plasticity
Hippocampus
Calcium Signaling
Gene Regulatory Networks
Quantitative Trait Loci
Reproductive Health
Muscle Contraction
Neuropeptides
Hypothalamus
Fear
Myocardium

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Exploring the involvement of Tac2 in the mouse hippocampal stress response through gene networking. / Hook, Mike; Xu, Fuyi; Terenina, Elena; Zhao, Wenyuan; Davenport, Athena; Mormede, Pierre; Jones, Byron; Mulligan, Megan; Lu, Lu.

In: Gene, Vol. 696, 15.05.2019, p. 176-185.

Research output: Contribution to journalArticle

Hook, Mike ; Xu, Fuyi ; Terenina, Elena ; Zhao, Wenyuan ; Davenport, Athena ; Mormede, Pierre ; Jones, Byron ; Mulligan, Megan ; Lu, Lu. / Exploring the involvement of Tac2 in the mouse hippocampal stress response through gene networking. In: Gene. 2019 ; Vol. 696. pp. 176-185.
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