Exposure of astrocytes to hypoxia/reoxygenation enhances expression of glucose-regulated protein 78 facilitating astrocyte release of the neuroprotective cytokine interleukin 6

Osamu Hori, Masayasu Matsumoto, Keisuke Kuwabara, Yusuke Maeda, Hirokazu Ueda, Toshiho Ohtsuki, Taroh Kinoshita, Satoshi Ogawa, David Stern, Takenobu Kamada

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Astrocytes exposed to hypoxia (H) or hypoxia/reoxygenation (H/R) maintain cell viability and display changes in protein biosynthesis. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of metabolically labeled astrocytes exposed to H showed induction of an ≃78-kDa polypeptide that demonstrated sequence identity with glucose-regulated protein (GRP) 78. Cell lysates from H/R astrocytes displayed induction of neuroprotectire interleukin (IL) 6, which was present in a high-molecular-weight complex also containing GRP78, suggesting that GRP78 might be functioning as a chaperone during cellular stress consequent on H/R. Introduction of antisense oligonucleotide to GRP78 into astrocytes prevented expression of the protein and suppressed H/R-induced astrocyte release of IL-6 by ≃50%. These data indicate that modulation of astrocyte properties during oxygen deprivation results, in part, from intracellular glucose depletion and subsequent expression of GRP78, which sustains generation of neuroproteotive IL-6 under the stress of H/R.

Original languageEnglish (US)
Pages (from-to)973-979
Number of pages7
JournalJournal of Neurochemistry
Volume66
Issue number3
StatePublished - Mar 1 1996
Externally publishedYes

Fingerprint

Astrocytes
Interleukin-6
Cytokines
Cell Hypoxia
Antisense Oligonucleotides
Biosynthesis
Protein Biosynthesis
Electrophoresis
Sodium Dodecyl Sulfate
glucose-regulated proteins
Hypoxia
Polyacrylamide Gel Electrophoresis
Cell Survival
Proteins
Molecular Weight
Molecular weight
Display devices
Cells
Modulation
Oxygen

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Exposure of astrocytes to hypoxia/reoxygenation enhances expression of glucose-regulated protein 78 facilitating astrocyte release of the neuroprotective cytokine interleukin 6. / Hori, Osamu; Matsumoto, Masayasu; Kuwabara, Keisuke; Maeda, Yusuke; Ueda, Hirokazu; Ohtsuki, Toshiho; Kinoshita, Taroh; Ogawa, Satoshi; Stern, David; Kamada, Takenobu.

In: Journal of Neurochemistry, Vol. 66, No. 3, 01.03.1996, p. 973-979.

Research output: Contribution to journalArticle

Hori, O, Matsumoto, M, Kuwabara, K, Maeda, Y, Ueda, H, Ohtsuki, T, Kinoshita, T, Ogawa, S, Stern, D & Kamada, T 1996, 'Exposure of astrocytes to hypoxia/reoxygenation enhances expression of glucose-regulated protein 78 facilitating astrocyte release of the neuroprotective cytokine interleukin 6', Journal of Neurochemistry, vol. 66, no. 3, pp. 973-979.
Hori, Osamu ; Matsumoto, Masayasu ; Kuwabara, Keisuke ; Maeda, Yusuke ; Ueda, Hirokazu ; Ohtsuki, Toshiho ; Kinoshita, Taroh ; Ogawa, Satoshi ; Stern, David ; Kamada, Takenobu. / Exposure of astrocytes to hypoxia/reoxygenation enhances expression of glucose-regulated protein 78 facilitating astrocyte release of the neuroprotective cytokine interleukin 6. In: Journal of Neurochemistry. 1996 ; Vol. 66, No. 3. pp. 973-979.
@article{50502fc2cb124535b1dcff0beebb3347,
title = "Exposure of astrocytes to hypoxia/reoxygenation enhances expression of glucose-regulated protein 78 facilitating astrocyte release of the neuroprotective cytokine interleukin 6",
abstract = "Astrocytes exposed to hypoxia (H) or hypoxia/reoxygenation (H/R) maintain cell viability and display changes in protein biosynthesis. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of metabolically labeled astrocytes exposed to H showed induction of an ≃78-kDa polypeptide that demonstrated sequence identity with glucose-regulated protein (GRP) 78. Cell lysates from H/R astrocytes displayed induction of neuroprotectire interleukin (IL) 6, which was present in a high-molecular-weight complex also containing GRP78, suggesting that GRP78 might be functioning as a chaperone during cellular stress consequent on H/R. Introduction of antisense oligonucleotide to GRP78 into astrocytes prevented expression of the protein and suppressed H/R-induced astrocyte release of IL-6 by ≃50{\%}. These data indicate that modulation of astrocyte properties during oxygen deprivation results, in part, from intracellular glucose depletion and subsequent expression of GRP78, which sustains generation of neuroproteotive IL-6 under the stress of H/R.",
author = "Osamu Hori and Masayasu Matsumoto and Keisuke Kuwabara and Yusuke Maeda and Hirokazu Ueda and Toshiho Ohtsuki and Taroh Kinoshita and Satoshi Ogawa and David Stern and Takenobu Kamada",
year = "1996",
month = "3",
day = "1",
language = "English (US)",
volume = "66",
pages = "973--979",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Exposure of astrocytes to hypoxia/reoxygenation enhances expression of glucose-regulated protein 78 facilitating astrocyte release of the neuroprotective cytokine interleukin 6

AU - Hori, Osamu

AU - Matsumoto, Masayasu

AU - Kuwabara, Keisuke

AU - Maeda, Yusuke

AU - Ueda, Hirokazu

AU - Ohtsuki, Toshiho

AU - Kinoshita, Taroh

AU - Ogawa, Satoshi

AU - Stern, David

AU - Kamada, Takenobu

PY - 1996/3/1

Y1 - 1996/3/1

N2 - Astrocytes exposed to hypoxia (H) or hypoxia/reoxygenation (H/R) maintain cell viability and display changes in protein biosynthesis. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of metabolically labeled astrocytes exposed to H showed induction of an ≃78-kDa polypeptide that demonstrated sequence identity with glucose-regulated protein (GRP) 78. Cell lysates from H/R astrocytes displayed induction of neuroprotectire interleukin (IL) 6, which was present in a high-molecular-weight complex also containing GRP78, suggesting that GRP78 might be functioning as a chaperone during cellular stress consequent on H/R. Introduction of antisense oligonucleotide to GRP78 into astrocytes prevented expression of the protein and suppressed H/R-induced astrocyte release of IL-6 by ≃50%. These data indicate that modulation of astrocyte properties during oxygen deprivation results, in part, from intracellular glucose depletion and subsequent expression of GRP78, which sustains generation of neuroproteotive IL-6 under the stress of H/R.

AB - Astrocytes exposed to hypoxia (H) or hypoxia/reoxygenation (H/R) maintain cell viability and display changes in protein biosynthesis. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of metabolically labeled astrocytes exposed to H showed induction of an ≃78-kDa polypeptide that demonstrated sequence identity with glucose-regulated protein (GRP) 78. Cell lysates from H/R astrocytes displayed induction of neuroprotectire interleukin (IL) 6, which was present in a high-molecular-weight complex also containing GRP78, suggesting that GRP78 might be functioning as a chaperone during cellular stress consequent on H/R. Introduction of antisense oligonucleotide to GRP78 into astrocytes prevented expression of the protein and suppressed H/R-induced astrocyte release of IL-6 by ≃50%. These data indicate that modulation of astrocyte properties during oxygen deprivation results, in part, from intracellular glucose depletion and subsequent expression of GRP78, which sustains generation of neuroproteotive IL-6 under the stress of H/R.

UR - http://www.scopus.com/inward/record.url?scp=13244300647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13244300647&partnerID=8YFLogxK

M3 - Article

VL - 66

SP - 973

EP - 979

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 3

ER -