Exposure to environmentally persistent free radicals during gestation lowers energy expenditure and impairs skeletal muscle mitochondrial function in adult mice

Erin Stephenson, Alyse Ragauskas, Sridhar Jaligama, Jeanna R. Redd, Jyothi Parvathareddy, Matthew J. Peloquin, Jordy Saravia, Joan Han, Stephania Cormier, Dave Bridges

Research output: Contribution to journalArticle

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Abstract

We have investigated the effects of in utero exposure to environmentally persistent free radicals (EPFRs) on growth, metabolism, energy utilization, and skeletal muscle mitochondria in a mouse model of diet-induced obesity. Pregnant mice were treated with laboratory-generated, combustion derived particular matter (MCP230). The adult offspring were placed on a high-fat diet for 12 wk, after which we observed a 9.8% increase in their body weight. The increase in body size observed in the MCP230-exposed mice was not associated with increases in food intake but was associated with a reduction in physical activity and lower energy expenditure. The reduced energy expenditure in mice indirectly exposed to MCP230 was associated with reductions in skeletal muscle mitochondrial DNA copy number, lower mRNA levels of electron transport genes, and reduced citrate synthase activity. Upregulation of key genes involved in ameliorating oxidative stress was also observed in the muscle of MCP230-exposed mice. These findings suggest that gestational exposure to MCP230 leads to a reduction in energy expenditure at least in part through alterations to mitochondrial metabolism in the skeletal muscle.

Original languageEnglish (US)
Pages (from-to)E1003-E1015
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume310
Issue number11
DOIs
StatePublished - Jun 1 2016

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Energy Metabolism
Free Radicals
Skeletal Muscle
Pregnancy
Muscle Mitochondrion
Citrate (si)-Synthase
Body Size
High Fat Diet
Electron Transport
Mitochondrial DNA
Genes
Oxidative Stress
Up-Regulation
Obesity
Eating
Body Weight
Diet
Muscles
Messenger RNA
Growth

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Exposure to environmentally persistent free radicals during gestation lowers energy expenditure and impairs skeletal muscle mitochondrial function in adult mice. / Stephenson, Erin; Ragauskas, Alyse; Jaligama, Sridhar; Redd, Jeanna R.; Parvathareddy, Jyothi; Peloquin, Matthew J.; Saravia, Jordy; Han, Joan; Cormier, Stephania; Bridges, Dave.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 310, No. 11, 01.06.2016, p. E1003-E1015.

Research output: Contribution to journalArticle

Stephenson, Erin ; Ragauskas, Alyse ; Jaligama, Sridhar ; Redd, Jeanna R. ; Parvathareddy, Jyothi ; Peloquin, Matthew J. ; Saravia, Jordy ; Han, Joan ; Cormier, Stephania ; Bridges, Dave. / Exposure to environmentally persistent free radicals during gestation lowers energy expenditure and impairs skeletal muscle mitochondrial function in adult mice. In: American Journal of Physiology - Endocrinology and Metabolism. 2016 ; Vol. 310, No. 11. pp. E1003-E1015.
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AU - Peloquin, Matthew J.

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