Expression analysis of melatonin receptor subtypes in the ovary of domestic chicken

N. R. Sundaresan, Marie Dennis Leo, J. Subramani, D. Anish, M. Sudhagar, K. A. Ahmed, M. Saxena, J. S. Tyagi, K. V.H. Sastry, V. K. Saxena

Research output: Contribution to journalArticle

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Abstract

Melatonin (N-acetyl-5-methoxytryptamine), an indole hormone, regulates various biological functions through three different receptor subtypes (Mel-1a, Mel-1b, and Mel-1c). However, the distribution of different melatonin receptor subtypes in chicken reproductive tissues was not known. In the present investigation, the partial sequences of ovarian melatonin receptor subtypes (Mel-1a, Mel-1b, and Mel-1c) were characterized. Further, the expression profile of melatonin receptor subtypes in the granulosa and theca layers of different preovulatory and postovulatory follicles (POF) were studied by semi-quantitative RT-PCR. The expression of all three subtypes of melatonin receptors were observed in the ovary of domestic chicken. Analysis of partial sequences of ovarian melatonin receptors revealed that the melatonin subtypes were identical to the brain receptors. In small white ovary follicles, we observed only the expression of mel-1b receptors, but not mel-1a or mel-1c receptors. In yellow follicles, all the three subtypes of receptors expression were noticed. Interestingly, we observed the expression of mel-1a receptor only in thecal layer, but not in granulosa layer. In contrast, mel-1b and -1c receptors were expressed in both granulosa and thecal layer. During the regression of POF, we observed significant upregulation of melatonin receptors (mel-1a and 1c) expression, that downregulated in the later stages of regression. We assume that the expression of melatonin receptors might have been influenced by the atresia or apoptosis of different follicular layers in POF. Our findings suggest that the differential distribution of melatonin receptor subtypes might have distinct downstream cellular functions in the ovarian tissues.

Original languageEnglish (US)
Pages (from-to)49-56
Number of pages8
JournalVeterinary Research Communications
Volume33
Issue number1
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

Fingerprint

Melatonin Receptors
melatonin
Ovary
Chickens
chickens
receptors
Melatonin MT2 Receptor
5-Methoxytryptamine
Melatonin
Sequence Analysis
Up-Regulation
Down-Regulation
abnormal development
Hormones
indoles

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Sundaresan, N. R., Leo, M. D., Subramani, J., Anish, D., Sudhagar, M., Ahmed, K. A., ... Saxena, V. K. (2009). Expression analysis of melatonin receptor subtypes in the ovary of domestic chicken. Veterinary Research Communications, 33(1), 49-56. https://doi.org/10.1007/s11259-008-9071-9

Expression analysis of melatonin receptor subtypes in the ovary of domestic chicken. / Sundaresan, N. R.; Leo, Marie Dennis; Subramani, J.; Anish, D.; Sudhagar, M.; Ahmed, K. A.; Saxena, M.; Tyagi, J. S.; Sastry, K. V.H.; Saxena, V. K.

In: Veterinary Research Communications, Vol. 33, No. 1, 01.01.2009, p. 49-56.

Research output: Contribution to journalArticle

Sundaresan, NR, Leo, MD, Subramani, J, Anish, D, Sudhagar, M, Ahmed, KA, Saxena, M, Tyagi, JS, Sastry, KVH & Saxena, VK 2009, 'Expression analysis of melatonin receptor subtypes in the ovary of domestic chicken', Veterinary Research Communications, vol. 33, no. 1, pp. 49-56. https://doi.org/10.1007/s11259-008-9071-9
Sundaresan, N. R. ; Leo, Marie Dennis ; Subramani, J. ; Anish, D. ; Sudhagar, M. ; Ahmed, K. A. ; Saxena, M. ; Tyagi, J. S. ; Sastry, K. V.H. ; Saxena, V. K. / Expression analysis of melatonin receptor subtypes in the ovary of domestic chicken. In: Veterinary Research Communications. 2009 ; Vol. 33, No. 1. pp. 49-56.
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