Expression and catalytic properties of rat liver NADP-isocitrate dehydrogenase under normal conditions, after administration of tumor necrosis factor-α and at thioctic acid action

L. N. Tsvetikova, T. N. Popova, T. I. Rakhmanova, Igor Iskusnykh

Research output: Contribution to journalArticle

Abstract

Development of apoptosis is accompanied by a decrease in transcripts of NADP-isocitrate dehydrogenase (NADP-IDH, E.C. 1.1.1.42.), and also alterations of catalytic properties from the rat liver enzyme in comparison with control. Administration of thioctic acid is increased the level of expression towards normal values. Enzyme preparations NADP-IDH were obtained from rat liver at norm conditions, after introduction of tumor necrosis factor and thioctic acid action under apoptosis. Molecular weight of homogenous preparations of NADP-IDH purified from livers of control and experimental rats was 112±5,8 kDa, however Km values and pH-optimum changed in apoptosis. Regulation of NADP-IDH activity under effect of some intermediates of the tricarboxylic acid cycle also differed in these groups.

Original languageEnglish (US)
Pages (from-to)220-229
Number of pages10
JournalBiomeditsinskaya Khimiya
Volume56
Issue number2
DOIs
StatePublished - Jan 1 2010

Fingerprint

Thioctic Acid
NADP
Liver
Rats
Tumor Necrosis Factor-alpha
Apoptosis
Citric Acid Cycle
Enzymes
Reference Values
Molecular Weight
Molecular weight
isocitrate dehydrogenase (NADP+)

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Expression and catalytic properties of rat liver NADP-isocitrate dehydrogenase under normal conditions, after administration of tumor necrosis factor-α and at thioctic acid action. / Tsvetikova, L. N.; Popova, T. N.; Rakhmanova, T. I.; Iskusnykh, Igor.

In: Biomeditsinskaya Khimiya, Vol. 56, No. 2, 01.01.2010, p. 220-229.

Research output: Contribution to journalArticle

@article{33ae9b5dfd6c4108ad52fa49babe813a,
title = "Expression and catalytic properties of rat liver NADP-isocitrate dehydrogenase under normal conditions, after administration of tumor necrosis factor-α and at thioctic acid action",
abstract = "Development of apoptosis is accompanied by a decrease in transcripts of NADP-isocitrate dehydrogenase (NADP-IDH, E.C. 1.1.1.42.), and also alterations of catalytic properties from the rat liver enzyme in comparison with control. Administration of thioctic acid is increased the level of expression towards normal values. Enzyme preparations NADP-IDH were obtained from rat liver at norm conditions, after introduction of tumor necrosis factor and thioctic acid action under apoptosis. Molecular weight of homogenous preparations of NADP-IDH purified from livers of control and experimental rats was 112±5,8 kDa, however Km values and pH-optimum changed in apoptosis. Regulation of NADP-IDH activity under effect of some intermediates of the tricarboxylic acid cycle also differed in these groups.",
author = "Tsvetikova, {L. N.} and Popova, {T. N.} and Rakhmanova, {T. I.} and Igor Iskusnykh",
year = "2010",
month = "1",
day = "1",
doi = "10.18097/pbmc20105602220",
language = "English (US)",
volume = "56",
pages = "220--229",
journal = "Biomeditsinskaya Khimiya",
issn = "2310-6905",
publisher = "Rossiiskaya Akademiya Meditsinskikh Nauk",
number = "2",

}

TY - JOUR

T1 - Expression and catalytic properties of rat liver NADP-isocitrate dehydrogenase under normal conditions, after administration of tumor necrosis factor-α and at thioctic acid action

AU - Tsvetikova, L. N.

AU - Popova, T. N.

AU - Rakhmanova, T. I.

AU - Iskusnykh, Igor

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Development of apoptosis is accompanied by a decrease in transcripts of NADP-isocitrate dehydrogenase (NADP-IDH, E.C. 1.1.1.42.), and also alterations of catalytic properties from the rat liver enzyme in comparison with control. Administration of thioctic acid is increased the level of expression towards normal values. Enzyme preparations NADP-IDH were obtained from rat liver at norm conditions, after introduction of tumor necrosis factor and thioctic acid action under apoptosis. Molecular weight of homogenous preparations of NADP-IDH purified from livers of control and experimental rats was 112±5,8 kDa, however Km values and pH-optimum changed in apoptosis. Regulation of NADP-IDH activity under effect of some intermediates of the tricarboxylic acid cycle also differed in these groups.

AB - Development of apoptosis is accompanied by a decrease in transcripts of NADP-isocitrate dehydrogenase (NADP-IDH, E.C. 1.1.1.42.), and also alterations of catalytic properties from the rat liver enzyme in comparison with control. Administration of thioctic acid is increased the level of expression towards normal values. Enzyme preparations NADP-IDH were obtained from rat liver at norm conditions, after introduction of tumor necrosis factor and thioctic acid action under apoptosis. Molecular weight of homogenous preparations of NADP-IDH purified from livers of control and experimental rats was 112±5,8 kDa, however Km values and pH-optimum changed in apoptosis. Regulation of NADP-IDH activity under effect of some intermediates of the tricarboxylic acid cycle also differed in these groups.

UR - http://www.scopus.com/inward/record.url?scp=77952969406&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952969406&partnerID=8YFLogxK

U2 - 10.18097/pbmc20105602220

DO - 10.18097/pbmc20105602220

M3 - Article

VL - 56

SP - 220

EP - 229

JO - Biomeditsinskaya Khimiya

JF - Biomeditsinskaya Khimiya

SN - 2310-6905

IS - 2

ER -