Expression and distribution of the dentatorubral-pallidoluysian atrophy gene product (atrophin-1/drplap) in neuronal and non-neuronal tissues

Steven P. Knight, Matthew M. Richardson, Alexander P. Osmand, Anna Stakkestad, Nicholas Potter

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Utilizing an affinity-purified antiserum directed against the carboxyl terminal region of atrophin-1/drplap (residues 1170-1185), we have examined the expression and distribution of the protein in a variety of neuronal and non-neuronal tissues. Immunohistochemical analyses of gelatin-embedded sections of monkey brain demonstrated a wide-spread distribution of the protein throughout the cerebrum and cerebellum. Labeling was primarily cytoplasmic within neuronal cell bodies and dendrites. Prominently staining regions included layers II, III, V, and VI of cerebral cortex, CA1-4 of the hippocampus, caudate nucleus, putamen, globus pallidus, amygdala, thalamus, red nucleus, pons, Purkinje cells, and deep cerebellar nuclei. Immunoblot analysis of extracts of frontal cortex from a wide variety of mammalian species (human, monkey, rabbit, rat, mouse, and bovine) detected a 190 kDa band in each extract. No cross-reactive material of similar molecular weight was detected in an extract of avian (chicken) central nervous system (CNS) tissue. Furthermore, in the rat, expression of the protein was predominantly neuronal in origin as immunoblot analyses of non-neuronal tissue extracts detected little or no 190 kDa protein. Collectively these investigations suggest a ubiquitous expression of atrophin-1/drplap in mammalian CNS tissue and provide initial immunochemical data for the study of the neuroanatomic and perhaps, phylogenetic relationships between mammalian and non-mammalian forms of the protein.

Original languageEnglish (US)
Pages (from-to)19-26
Number of pages8
JournalJournal of the Neurological Sciences
Volume146
Issue number1
DOIs
StatePublished - Feb 27 1997
Externally publishedYes

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Progressive Myoclonic Epilepsy
Nerve Tissue
Genes
Haplorhini
Proteins
Central Nervous System
Red Nucleus
Cerebellar Nuclei
Globus Pallidus
Tissue Extracts
Pons
Caudate Nucleus
Purkinje Cells
Putamen
Cerebrum
Frontal Lobe
Gelatin
Dendrites
Amygdala
Thalamus

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Expression and distribution of the dentatorubral-pallidoluysian atrophy gene product (atrophin-1/drplap) in neuronal and non-neuronal tissues. / Knight, Steven P.; Richardson, Matthew M.; Osmand, Alexander P.; Stakkestad, Anna; Potter, Nicholas.

In: Journal of the Neurological Sciences, Vol. 146, No. 1, 27.02.1997, p. 19-26.

Research output: Contribution to journalArticle

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