Expression and localization of angiogenin in placenta

Enhanced levels at term over first trimester villi

Raja Shekhar Gangaraju, A. Loganath, A. C. Roy, Y. C. Wong

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Human angiogenin, a 14-kDa non-glycosylated polypeptide with both angiogenic and ribonucleolytic activities, is implicated in angiogenesis, a complex process of proliferation and formation of new capillary blood vessels from existing blood vessels. Placental growth requires extensive angiogenesis, which develops its vascular structure in both fetal chorionic villi and maternal deciduas. In this study, we investigated the expression profiles of angiogenin in placental villi from early and late gestation at both mRNA and protein levels using explant cultures in vitro followed by RT-PCR, immunoblot, and immunohistochemical analyses. From functionally active placental explants, angiogenin was detected in conditioned media of all the samples from first trimester and term group. The mean levels of angiogenin produced by term villi were found to be 2.6-, 2.1-, and 2.2-fold higher (P < 0.01) than first trimester villi at 24, 48, and 72 hr of culture, respectively. Expression profiles of angiogenin from term and first trimester villi seem to agree with its mRNA levels and immunoblot analysis; the expression in term villi was twice that in first trimester villi. The presence of angiogenin in placental villi and upregulation of its production towards term indicate that angiogenin production by the placenta is specific to the developmental stage. In conclusion, the observed changes in the localization and mRNA expression of angiogenin during placental development raise the possibility that it is involved in morphological and angiogenic changes in this endocrine organ vital to the successful fetal outcome during pregnancy.

Original languageEnglish (US)
Pages (from-to)159-166
Number of pages8
JournalMolecular Reproduction and Development
Volume62
Issue number2
DOIs
StatePublished - May 20 2002
Externally publishedYes

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First Pregnancy Trimester
Placenta
Chorionic Villi
Blood Vessels
Messenger RNA
Placentation
angiogenin
Pregnancy Outcome
Conditioned Culture Medium
Up-Regulation
Mothers
Pregnancy
Polymerase Chain Reaction
Peptides
Growth

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Expression and localization of angiogenin in placenta : Enhanced levels at term over first trimester villi. / Gangaraju, Raja Shekhar; Loganath, A.; Roy, A. C.; Wong, Y. C.

In: Molecular Reproduction and Development, Vol. 62, No. 2, 20.05.2002, p. 159-166.

Research output: Contribution to journalArticle

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