Expression Levels of Obesity-Related Genes Are Associated with Weight Change in Kidney Transplant Recipients

Ann Cashion, Ansley Stanfill, Fridtjof Thomas, Lijing Xu, Thomas Sutter, James Eason, Mang Ensell, Ramin Homayouni

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: The aim of this study was to investigate the association of gene expression profiles in subcutaneous adipose tissue with weight change in kidney transplant recipients and to gain insights into the underlying mechanisms of weight gain. Methodology/Principal Findings: A secondary data analysis was done on a subgroup (n = 26) of existing clinical and gene expression data from a larger prospective longitudinal study examining factors contributing to weight gain in transplant recipients. Measurements taken included adipose tissue gene expression profiles at time of transplant, baseline and six-month weight, and demographic data. Using multivariate linear regression analysis controlled for race and gender, expression levels of 1553 genes were significantly (p<0.05) associated with weight change. Functional analysis using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes classifications identified metabolic pathways that were enriched in this dataset. Furthermore, GeneIndexer literature mining analysis identified a subset of genes that are highly associated with obesity in the literature and Ingenuity pathway analysis revealed several significant gene networks associated with metabolism and endocrine function. Polymorphisms in several of these genes have previously been linked to obesity. Conclusions/Significance: We have successfully identified a set of molecular pathways that taken together may provide insights into the mechanisms of weight gain in kidney transplant recipients. Future work will be done to determine how these pathways may contribute to weight gain.

Original languageEnglish (US)
Article numbere59962
JournalPLoS One
Volume8
Issue number3
DOIs
StatePublished - Mar 27 2013

Fingerprint

kidney transplant
Transplants
Weight Gain
obesity
Obesity
Genes
Kidney
Weights and Measures
weight gain
Transcriptome
Gene expression
genes
gene expression
adipose tissue
Encyclopedias
Gene Ontology
Gene Regulatory Networks
Subcutaneous Fat
Metabolic Networks and Pathways
Longitudinal Studies

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Expression Levels of Obesity-Related Genes Are Associated with Weight Change in Kidney Transplant Recipients. / Cashion, Ann; Stanfill, Ansley; Thomas, Fridtjof; Xu, Lijing; Sutter, Thomas; Eason, James; Ensell, Mang; Homayouni, Ramin.

In: PLoS One, Vol. 8, No. 3, e59962, 27.03.2013.

Research output: Contribution to journalArticle

Cashion, Ann ; Stanfill, Ansley ; Thomas, Fridtjof ; Xu, Lijing ; Sutter, Thomas ; Eason, James ; Ensell, Mang ; Homayouni, Ramin. / Expression Levels of Obesity-Related Genes Are Associated with Weight Change in Kidney Transplant Recipients. In: PLoS One. 2013 ; Vol. 8, No. 3.
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