Expression of α V β 3 integrin in rat endometrial epithelial cells and its functional role during implantation

Kokatam R. Srinivasan, Chellakkan S. Blesson, Iram Fatima, Shakti Kitchlu, Swatantra K. Jain, Purshottam K. Mehrotra, Anila Dwivedi

Research output: Contribution to journalArticle

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Abstract

The α V β 3 integrin as a marker of endometrial receptivity has been well established in human and other mammalian species; however, its expression is still not known in rats. Our objective was to establish the expression of α V β 3 integrin as a marker of endometrial receptivity in rat and to further prove its role in implantation by function-blocking studies in this species. Immunocytochemical, immunohistochemical and flow-cytometric studies were performed in rat endometrial epithelial cells (EEC) to demonstrate the expression of α V β 3 integrin during non-receptive, pre-receptive and receptive phases of the uterus. Results revealed positive immunocytochemical staining for α v and β 3 subunits on the surface of EEC of days 4 and 5 p.c. (post-coitum), but the intensity was higher in cells of day 5 p.c. Flow-cytometric study revealed higher level of α V β 3 on day 5 p.c. as compared to day 4 p.c. and non-pregnant animals. Immunohistochemical analysis of uterine tissue also revealed that the α V β 3 expression in LE was higher on day 5 p.c. morning as compared to that observed on day 4 p.c. In addition, the expression of β 3 subunit was not evident in rats receiving ormeloxifene, an agent known to inhibit the uterine receptivity. Immunoblotting experiments also revealed higher expression of uterine β 3 on day 5 p.c. On day 6, expression of β 3 was high in implantation sites than on inter-implantation sites. In immature ovariectomized rats, α V β 3 was up-regulated by progesterone and by a combination of estrogen and progesterone. The expression of α V β 3 was also up-regulated in EEC co-cultured with blastocysts. All the agents used for function-blocking studies showed significant reduction in the number of implantation sites in treated horn as compared to sham control horn. The present study has successfully demonstrated the expression of α V β 3 in rat EEC as a marker of endometrial receptivity and showed that this molecule is indispensable for the process of implantation in this species.

Original languageEnglish (US)
Pages (from-to)124-133
Number of pages10
JournalGeneral and Comparative Endocrinology
Volume160
Issue number2
DOIs
StatePublished - Jan 15 2009

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integrins
Integrins
epithelial cells
Epithelial Cells
rats
Horns
Progesterone
progesterone
uterine tissue
Blastocyst
immunoblotting
Immunoblotting
blastocyst
uterus
estrogens
Uterus
Estrogens
immatures
Staining and Labeling
animals

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Endocrinology

Cite this

Expression of α V β 3 integrin in rat endometrial epithelial cells and its functional role during implantation . / Srinivasan, Kokatam R.; Blesson, Chellakkan S.; Fatima, Iram; Kitchlu, Shakti; Jain, Swatantra K.; Mehrotra, Purshottam K.; Dwivedi, Anila.

In: General and Comparative Endocrinology, Vol. 160, No. 2, 15.01.2009, p. 124-133.

Research output: Contribution to journalArticle

Srinivasan, Kokatam R. ; Blesson, Chellakkan S. ; Fatima, Iram ; Kitchlu, Shakti ; Jain, Swatantra K. ; Mehrotra, Purshottam K. ; Dwivedi, Anila. / Expression of α V β 3 integrin in rat endometrial epithelial cells and its functional role during implantation In: General and Comparative Endocrinology. 2009 ; Vol. 160, No. 2. pp. 124-133.
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AU - Kitchlu, Shakti

AU - Jain, Swatantra K.

AU - Mehrotra, Purshottam K.

AU - Dwivedi, Anila

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