Expression of ᵞ-Glutamylcysteine Synthetase in the Liver of Copper-Deficient Rats

Yan Chen, Yujian Kang, Jack T. Saari

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Copper deficiency in rats increases hepatic glutathione concentration. The present study was undertaken to determine the biochemical and molecular basis for the glutathione elevation. Weanling Sprague-Dawley rats were fed a purified diet deficient in copper (0.4 μg/g diet) or one containing adequate copper (5.7 μg/g diet) for 4 weeks. Hepatic glutathione concentration, the activity of the rate-limiting enzyme in glutathione biosynthesis, γ-glutamylcysteine synthetase (γ-GCS), and the relative amount of mRNA for the enzyme were determined. Hepatic glutathione concentration in copper-deficient rats was significantly elevated (6.6 vs 5.6 μmol/g). The activity of hepatic γ-GCS was 1.6 times higher in the copper-deficient than in the copper-adequate rats (58.0 vs 35.9 nmol NADH/min · mg protein). The steady-state amount of mRNA for γ-GCS was increased 5-fold in the copper-deficient rat liver. The findings demonstrate that the elevated hepatic glutathione concentration in copper-deficient rats results from upregulation of γ-GCS activity. This study provides further understanding of changes in hepatic glutathione metabolism induced by copper deficiency.

Original languageEnglish (US)
Pages (from-to)102-106
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume210
Issue number2
DOIs
StatePublished - Jan 1 1995

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Glutamate-Cysteine Ligase
Liver
Rats
Copper
Glutathione
Nutrition
Diet
Messenger RNA
Biosynthesis
Enzymes
Metabolism
NAD
Sprague Dawley Rats
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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Expression of ᵞ-Glutamylcysteine Synthetase in the Liver of Copper-Deficient Rats. / Chen, Yan; Kang, Yujian; Saari, Jack T.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 210, No. 2, 01.01.1995, p. 102-106.

Research output: Contribution to journalArticle

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