Expression of a metalloproteinase that degrades native type V collagen and denaturated collagens by cultured human alveolar macrophages

M. S. Hibbs, J. R. Hoidal, Andrew Kang

Research output: Contribution to journalArticle

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Abstract

Human pulmonary alveolar macrophages obtained by bronchoalveolar lavage from both normal controls and smokers secreted in vitro a neutral proteinase that degraded denaturated collagens. Optimal expression of the proteinase was detected after 3-5 d of culture. The proteinase could not be detected in the media of cultures that had been treated with 0.5 μg/ml of cycloheximide. The gelatinase had an M(r) of 90,000 and was immunologically cross-reactive with human neutrophil gelatinase. When newly synthesized 35S-methionine-labeled proteins were analyzed, the proteinase appeared to be a major secretion product of alveolar macrophages. Chromatography on gelatin-Sepharose gave a single peak of activity that was predominantly composed of the 90,000-mol-wt proteinase. The proteolytic activity in the gelatin-Sepharose-purified material was inhibited by EDTA and 1,10-phenanthroline, but not by N-ethylmaleimide or phenylmethanesulfonyl fluoride, indicating that the proteinase was a metalloproteinase. The partially purified material was also capable of degrading native type V collagen and this degradation was inhibited in the presence of an antibody to neutrophil gelatinase. The data suggest that human alveolar macrophages in culture elaborate a metalloproteinase that degrades both native type V collagen and denatured collagens.

Original languageEnglish (US)
Pages (from-to)1644-1650
Number of pages7
JournalJournal of Clinical Investigation
Volume80
Issue number6
DOIs
StatePublished - Jan 1 1987

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Collagen Type V
Alveolar Macrophages
Metalloproteases
Peptide Hydrolases
Collagen
Gelatinases
Gelatin
Sepharose
Neutrophils
Phenylmethylsulfonyl Fluoride
Ethylmaleimide
Bronchoalveolar Lavage
Cycloheximide
Edetic Acid
Methionine
Culture Media
Chromatography
Antibodies

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Expression of a metalloproteinase that degrades native type V collagen and denaturated collagens by cultured human alveolar macrophages. / Hibbs, M. S.; Hoidal, J. R.; Kang, Andrew.

In: Journal of Clinical Investigation, Vol. 80, No. 6, 01.01.1987, p. 1644-1650.

Research output: Contribution to journalArticle

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