Expression of a novel RNA-splicing factor, RA301/Tra2β, in vascular lesions and its role in smooth muscle cell proliferation

Yoshitane Tsukamoto, Noriyuki Matsuo, Kentaro Ozawa, Osamu Hori, Toshio Higashi, Junya Nishizaki, Norimitsu Tohnai, Izumi Nagata, Kiyoshi Kawano, Chikao Yutani, Seiichi Hirota, Yukihiko Kitamura, David Stern, Satoshi Ogawa

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

RA301/Tra2β, a sequence-specific RNA-binding protein, was first cloned as a stress molecule in re-oxygenated astrocytes. In human vascular tissues, we have found enhanced RA301/Tra2β expression in coronary artery with intimal thickening, and atherosclerotic aorta. Balloon injury to the rat carotid artery induced RA301/Tra2β transcripts followed by expression of the antigen, which was detected in medial and neointimal vascular smooth muscle cells (VSMCs). In cultured VSMCs, hypoxia/re-oxygenation caused induction of RA301/Tra2β and was accompanied by cell proliferation, both of which were blocked by the addition of either diphenyl iodonium, a NADPH oxidase inhibitor, PD98059, a mitogen-activated protein kinase kinase inhibitor, or antisense oligonucleotide for RA301/Tra2β. Consistent with a link between RA301/Tra2β and cell proliferation, platelet-derived growth factor also induced expression of RA301/ Tra2β in cultured VSMCs. These data suggest a possible role for RA301/Tra2β in the regulation of VSMC proliferation, especially in the setting of hypoxia/reoxygenation-induced cell stress.

Original languageEnglish (US)
Pages (from-to)1685-1694
Number of pages10
JournalAmerican Journal of Pathology
Volume158
Issue number5
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

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Vascular Smooth Muscle
Smooth Muscle Myocytes
Blood Vessels
Cell Proliferation
Tunica Intima
Cell Hypoxia
RNA-Binding Proteins
Antisense Oligonucleotides
NADPH Oxidase
Platelet-Derived Growth Factor
Mitogen-Activated Protein Kinase Kinases
Carotid Arteries
Astrocytes
Aorta
Coronary Vessels
Antigens
RNA Splicing Factors
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Expression of a novel RNA-splicing factor, RA301/Tra2β, in vascular lesions and its role in smooth muscle cell proliferation. / Tsukamoto, Yoshitane; Matsuo, Noriyuki; Ozawa, Kentaro; Hori, Osamu; Higashi, Toshio; Nishizaki, Junya; Tohnai, Norimitsu; Nagata, Izumi; Kawano, Kiyoshi; Yutani, Chikao; Hirota, Seiichi; Kitamura, Yukihiko; Stern, David; Ogawa, Satoshi.

In: American Journal of Pathology, Vol. 158, No. 5, 01.01.2001, p. 1685-1694.

Research output: Contribution to journalArticle

Tsukamoto, Y, Matsuo, N, Ozawa, K, Hori, O, Higashi, T, Nishizaki, J, Tohnai, N, Nagata, I, Kawano, K, Yutani, C, Hirota, S, Kitamura, Y, Stern, D & Ogawa, S 2001, 'Expression of a novel RNA-splicing factor, RA301/Tra2β, in vascular lesions and its role in smooth muscle cell proliferation', American Journal of Pathology, vol. 158, no. 5, pp. 1685-1694. https://doi.org/10.1016/S0002-9440(10)64124-7
Tsukamoto, Yoshitane ; Matsuo, Noriyuki ; Ozawa, Kentaro ; Hori, Osamu ; Higashi, Toshio ; Nishizaki, Junya ; Tohnai, Norimitsu ; Nagata, Izumi ; Kawano, Kiyoshi ; Yutani, Chikao ; Hirota, Seiichi ; Kitamura, Yukihiko ; Stern, David ; Ogawa, Satoshi. / Expression of a novel RNA-splicing factor, RA301/Tra2β, in vascular lesions and its role in smooth muscle cell proliferation. In: American Journal of Pathology. 2001 ; Vol. 158, No. 5. pp. 1685-1694.
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AU - Nishizaki, Junya

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