Expression of an Nkx3.1-CRE gene using ROSA26 reporter mice

M. N. Stanfel, K. A. Moses, James Carson, D. B. Zimmer, F. DeMayo, R. J. Schwartz, Warren E. Zimmer

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The genetic locus of Nkx3.1, an early murine marker of sclerotome and prostate development, was disrupted by a knock in of CRE recombinase via homologous recombination in embryonic stem cells. Cell fate mapping revealed previously unidentified cell lineages expanded from Nkx3.1-expressing cell populations and recapitulated reported Nkx3.1 expression patterns. In lineage trace experiments of E18.5 Nkx3.1-CRE; R26R embryos novel staining was observed in areas of the lungs, portions of the duodenum, and vertebral elements of the skeleton. β-galactosidase activity measured in Nkx3.1-CRE; R26R and Nkx3.2-CRE; R26R embryos was observed in overlapping regions of the sclerotome but no apparent change in Nkx3.1 expression was seen in the Nkx3.2 mutants by in situ hybridization.

Original languageEnglish (US)
Pages (from-to)550-555
Number of pages6
JournalGenesis
Volume44
Issue number11
DOIs
StatePublished - Nov 1 2006

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Embryonic Structures
Galactosidases
Recombinases
Genetic Loci
Homologous Recombination
Cell Lineage
Embryonic Stem Cells
Duodenum
Skeleton
Genes
In Situ Hybridization
Prostate
Staining and Labeling
Lung
Population

All Science Journal Classification (ASJC) codes

  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Stanfel, M. N., Moses, K. A., Carson, J., Zimmer, D. B., DeMayo, F., Schwartz, R. J., & Zimmer, W. E. (2006). Expression of an Nkx3.1-CRE gene using ROSA26 reporter mice. Genesis, 44(11), 550-555. https://doi.org/10.1002/dvg.20250

Expression of an Nkx3.1-CRE gene using ROSA26 reporter mice. / Stanfel, M. N.; Moses, K. A.; Carson, James; Zimmer, D. B.; DeMayo, F.; Schwartz, R. J.; Zimmer, Warren E.

In: Genesis, Vol. 44, No. 11, 01.11.2006, p. 550-555.

Research output: Contribution to journalArticle

Stanfel, MN, Moses, KA, Carson, J, Zimmer, DB, DeMayo, F, Schwartz, RJ & Zimmer, WE 2006, 'Expression of an Nkx3.1-CRE gene using ROSA26 reporter mice', Genesis, vol. 44, no. 11, pp. 550-555. https://doi.org/10.1002/dvg.20250
Stanfel MN, Moses KA, Carson J, Zimmer DB, DeMayo F, Schwartz RJ et al. Expression of an Nkx3.1-CRE gene using ROSA26 reporter mice. Genesis. 2006 Nov 1;44(11):550-555. https://doi.org/10.1002/dvg.20250
Stanfel, M. N. ; Moses, K. A. ; Carson, James ; Zimmer, D. B. ; DeMayo, F. ; Schwartz, R. J. ; Zimmer, Warren E. / Expression of an Nkx3.1-CRE gene using ROSA26 reporter mice. In: Genesis. 2006 ; Vol. 44, No. 11. pp. 550-555.
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