Expression of c-kit and platelet-derived growth factor receptors in ovarian granulosa cell tumors

Rodney P. Rocconi, Kellie S. Matthews, Kristopher Kimball, Michael G. Conner, Allyson C. Baker, Mack N. Barnes

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective. This study aimed at evaluating the expression of tyrosine kinase receptors c-kit, (platelet-derived growth factor receptor-α (PDGFR-α), and PDGFR-β in ovarian granulosa cell tumors (GCTs). Study design. Primary ovarian GCT specimens were obtained for immunohistochemical staining.The expressions of c-kit, PDGFR-α, and PDGFR-β were analyzed and scored by a semiquantitative (SQ) method. Normal ovarian tissue from the same patients' specimens served as internal controls. Results. A total of 21 specimens were available for evaluation. C-kit was expressed in only 2 samples, whereas both PDGFR-α and PDGFR-β stained positive in 100% of tumors. PDGFR targets demonstrated strong positive expression in intensity and amount of tissue stained. Normal ovarian tissue demonstrated complete absence of staining for all 3 antibodies evaluated. Conclusions. The data demonstrated significant expression of PDGFR targets of imatinib mesylate in GCTs, whereas normal ovarian tissues had a complete absence of staining.This expression profile provides the rationale to investigate the role of imatinib mesylate in PDGFR-positive GCTs.

Original languageEnglish (US)
Pages (from-to)673-677
Number of pages5
JournalReproductive Sciences
Volume15
Issue number7
DOIs
StatePublished - Sep 1 2008

Fingerprint

Granulosa Cell Tumor
Platelet-Derived Growth Factor Receptors
Staining and Labeling
Receptor Protein-Tyrosine Kinases
Antibodies

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynecology

Cite this

Expression of c-kit and platelet-derived growth factor receptors in ovarian granulosa cell tumors. / Rocconi, Rodney P.; Matthews, Kellie S.; Kimball, Kristopher; Conner, Michael G.; Baker, Allyson C.; Barnes, Mack N.

In: Reproductive Sciences, Vol. 15, No. 7, 01.09.2008, p. 673-677.

Research output: Contribution to journalArticle

Rocconi, Rodney P. ; Matthews, Kellie S. ; Kimball, Kristopher ; Conner, Michael G. ; Baker, Allyson C. ; Barnes, Mack N. / Expression of c-kit and platelet-derived growth factor receptors in ovarian granulosa cell tumors. In: Reproductive Sciences. 2008 ; Vol. 15, No. 7. pp. 673-677.
@article{ab2e30d1308e44df9ab8324febb176ad,
title = "Expression of c-kit and platelet-derived growth factor receptors in ovarian granulosa cell tumors",
abstract = "Objective. This study aimed at evaluating the expression of tyrosine kinase receptors c-kit, (platelet-derived growth factor receptor-α (PDGFR-α), and PDGFR-β in ovarian granulosa cell tumors (GCTs). Study design. Primary ovarian GCT specimens were obtained for immunohistochemical staining.The expressions of c-kit, PDGFR-α, and PDGFR-β were analyzed and scored by a semiquantitative (SQ) method. Normal ovarian tissue from the same patients' specimens served as internal controls. Results. A total of 21 specimens were available for evaluation. C-kit was expressed in only 2 samples, whereas both PDGFR-α and PDGFR-β stained positive in 100{\%} of tumors. PDGFR targets demonstrated strong positive expression in intensity and amount of tissue stained. Normal ovarian tissue demonstrated complete absence of staining for all 3 antibodies evaluated. Conclusions. The data demonstrated significant expression of PDGFR targets of imatinib mesylate in GCTs, whereas normal ovarian tissues had a complete absence of staining.This expression profile provides the rationale to investigate the role of imatinib mesylate in PDGFR-positive GCTs.",
author = "Rocconi, {Rodney P.} and Matthews, {Kellie S.} and Kristopher Kimball and Conner, {Michael G.} and Baker, {Allyson C.} and Barnes, {Mack N.}",
year = "2008",
month = "9",
day = "1",
doi = "10.1177/1933719108317584",
language = "English (US)",
volume = "15",
pages = "673--677",
journal = "Reproductive Sciences",
issn = "1933-7191",
publisher = "SAGE Publications Inc.",
number = "7",

}

TY - JOUR

T1 - Expression of c-kit and platelet-derived growth factor receptors in ovarian granulosa cell tumors

AU - Rocconi, Rodney P.

AU - Matthews, Kellie S.

AU - Kimball, Kristopher

AU - Conner, Michael G.

AU - Baker, Allyson C.

AU - Barnes, Mack N.

PY - 2008/9/1

Y1 - 2008/9/1

N2 - Objective. This study aimed at evaluating the expression of tyrosine kinase receptors c-kit, (platelet-derived growth factor receptor-α (PDGFR-α), and PDGFR-β in ovarian granulosa cell tumors (GCTs). Study design. Primary ovarian GCT specimens were obtained for immunohistochemical staining.The expressions of c-kit, PDGFR-α, and PDGFR-β were analyzed and scored by a semiquantitative (SQ) method. Normal ovarian tissue from the same patients' specimens served as internal controls. Results. A total of 21 specimens were available for evaluation. C-kit was expressed in only 2 samples, whereas both PDGFR-α and PDGFR-β stained positive in 100% of tumors. PDGFR targets demonstrated strong positive expression in intensity and amount of tissue stained. Normal ovarian tissue demonstrated complete absence of staining for all 3 antibodies evaluated. Conclusions. The data demonstrated significant expression of PDGFR targets of imatinib mesylate in GCTs, whereas normal ovarian tissues had a complete absence of staining.This expression profile provides the rationale to investigate the role of imatinib mesylate in PDGFR-positive GCTs.

AB - Objective. This study aimed at evaluating the expression of tyrosine kinase receptors c-kit, (platelet-derived growth factor receptor-α (PDGFR-α), and PDGFR-β in ovarian granulosa cell tumors (GCTs). Study design. Primary ovarian GCT specimens were obtained for immunohistochemical staining.The expressions of c-kit, PDGFR-α, and PDGFR-β were analyzed and scored by a semiquantitative (SQ) method. Normal ovarian tissue from the same patients' specimens served as internal controls. Results. A total of 21 specimens were available for evaluation. C-kit was expressed in only 2 samples, whereas both PDGFR-α and PDGFR-β stained positive in 100% of tumors. PDGFR targets demonstrated strong positive expression in intensity and amount of tissue stained. Normal ovarian tissue demonstrated complete absence of staining for all 3 antibodies evaluated. Conclusions. The data demonstrated significant expression of PDGFR targets of imatinib mesylate in GCTs, whereas normal ovarian tissues had a complete absence of staining.This expression profile provides the rationale to investigate the role of imatinib mesylate in PDGFR-positive GCTs.

UR - http://www.scopus.com/inward/record.url?scp=52449083054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52449083054&partnerID=8YFLogxK

U2 - 10.1177/1933719108317584

DO - 10.1177/1933719108317584

M3 - Article

VL - 15

SP - 673

EP - 677

JO - Reproductive Sciences

JF - Reproductive Sciences

SN - 1933-7191

IS - 7

ER -