Expression of delta opioid receptors and transcripts by splenic T cells

Burt Sharp, Ming D. Li, Shannon G. Matta, Kathy McAllen, Nahid A. Shahabi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Delta opioid receptors (DORs) and preproenkephalin-A-derived opiate peptides are expressed by mononuclear cells in various lymphoid organs. DOR ligands modulate a variety of immune functions, such as T-cell proliferation, calcium mobilization, and cytokine production. Recently, quiescent T cells were found to express low levels of DOR transcripts, which increased due to the following: cell culture of unstimulated murine splenocytes (depending on cell density); cross-linking the T-cell receptor (TCR) with anti-CD3-ε; and a single in vivo exposure to staphylococcal enterotoxin B (SEB). Enhanced expression of DOR mRNA was mediated transcriptionally. Moreover, PMA + ionomycin, which mimic the proliferative signal of anti-CD3, inhibited the expression of DOR mRNA. Using semiquantitative immunofluorescence to detect DORs, SEB was found to increase the fraction of T cells that expressed DOR and to enhance the relative level of DOR expression per T cell. Previous studies have shown that DOR agonists inhibited the anti-CD3-stimulated production of interleukin-2 and T-cell proliferation. Therefore, the enhanced expression of DORs by activated T cells may be capable of downregulating the T-cell activation program.

Original languageEnglish (US)
Pages (from-to)764-770
Number of pages7
JournalAnnals of the New York Academy of Sciences
Volume917
StatePublished - Jan 1 2000

Fingerprint

delta Opioid Receptor
T-cells
T-Lymphocytes
Cell proliferation
Cell Proliferation
Cells
Messenger RNA
Ionomycin
Opioid Peptides
T-Cell Antigen Receptor
Cell culture
Interleukin-2
Fluorescent Antibody Technique
Down-Regulation
Cell Culture Techniques
Cell Count
Chemical activation
Cytokines
Ligands
Calcium

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Sharp, B., Li, M. D., Matta, S. G., McAllen, K., & Shahabi, N. A. (2000). Expression of delta opioid receptors and transcripts by splenic T cells. Annals of the New York Academy of Sciences, 917, 764-770.

Expression of delta opioid receptors and transcripts by splenic T cells. / Sharp, Burt; Li, Ming D.; Matta, Shannon G.; McAllen, Kathy; Shahabi, Nahid A.

In: Annals of the New York Academy of Sciences, Vol. 917, 01.01.2000, p. 764-770.

Research output: Contribution to journalArticle

Sharp, Burt ; Li, Ming D. ; Matta, Shannon G. ; McAllen, Kathy ; Shahabi, Nahid A. / Expression of delta opioid receptors and transcripts by splenic T cells. In: Annals of the New York Academy of Sciences. 2000 ; Vol. 917. pp. 764-770.
@article{16aeaa62f6c14a8d90b0c72096ee2a78,
title = "Expression of delta opioid receptors and transcripts by splenic T cells",
abstract = "Delta opioid receptors (DORs) and preproenkephalin-A-derived opiate peptides are expressed by mononuclear cells in various lymphoid organs. DOR ligands modulate a variety of immune functions, such as T-cell proliferation, calcium mobilization, and cytokine production. Recently, quiescent T cells were found to express low levels of DOR transcripts, which increased due to the following: cell culture of unstimulated murine splenocytes (depending on cell density); cross-linking the T-cell receptor (TCR) with anti-CD3-ε; and a single in vivo exposure to staphylococcal enterotoxin B (SEB). Enhanced expression of DOR mRNA was mediated transcriptionally. Moreover, PMA + ionomycin, which mimic the proliferative signal of anti-CD3, inhibited the expression of DOR mRNA. Using semiquantitative immunofluorescence to detect DORs, SEB was found to increase the fraction of T cells that expressed DOR and to enhance the relative level of DOR expression per T cell. Previous studies have shown that DOR agonists inhibited the anti-CD3-stimulated production of interleukin-2 and T-cell proliferation. Therefore, the enhanced expression of DORs by activated T cells may be capable of downregulating the T-cell activation program.",
author = "Burt Sharp and Li, {Ming D.} and Matta, {Shannon G.} and Kathy McAllen and Shahabi, {Nahid A.}",
year = "2000",
month = "1",
day = "1",
language = "English (US)",
volume = "917",
pages = "764--770",
journal = "Annals of the New York Academy of Sciences",
issn = "0077-8923",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Expression of delta opioid receptors and transcripts by splenic T cells

AU - Sharp, Burt

AU - Li, Ming D.

AU - Matta, Shannon G.

AU - McAllen, Kathy

AU - Shahabi, Nahid A.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Delta opioid receptors (DORs) and preproenkephalin-A-derived opiate peptides are expressed by mononuclear cells in various lymphoid organs. DOR ligands modulate a variety of immune functions, such as T-cell proliferation, calcium mobilization, and cytokine production. Recently, quiescent T cells were found to express low levels of DOR transcripts, which increased due to the following: cell culture of unstimulated murine splenocytes (depending on cell density); cross-linking the T-cell receptor (TCR) with anti-CD3-ε; and a single in vivo exposure to staphylococcal enterotoxin B (SEB). Enhanced expression of DOR mRNA was mediated transcriptionally. Moreover, PMA + ionomycin, which mimic the proliferative signal of anti-CD3, inhibited the expression of DOR mRNA. Using semiquantitative immunofluorescence to detect DORs, SEB was found to increase the fraction of T cells that expressed DOR and to enhance the relative level of DOR expression per T cell. Previous studies have shown that DOR agonists inhibited the anti-CD3-stimulated production of interleukin-2 and T-cell proliferation. Therefore, the enhanced expression of DORs by activated T cells may be capable of downregulating the T-cell activation program.

AB - Delta opioid receptors (DORs) and preproenkephalin-A-derived opiate peptides are expressed by mononuclear cells in various lymphoid organs. DOR ligands modulate a variety of immune functions, such as T-cell proliferation, calcium mobilization, and cytokine production. Recently, quiescent T cells were found to express low levels of DOR transcripts, which increased due to the following: cell culture of unstimulated murine splenocytes (depending on cell density); cross-linking the T-cell receptor (TCR) with anti-CD3-ε; and a single in vivo exposure to staphylococcal enterotoxin B (SEB). Enhanced expression of DOR mRNA was mediated transcriptionally. Moreover, PMA + ionomycin, which mimic the proliferative signal of anti-CD3, inhibited the expression of DOR mRNA. Using semiquantitative immunofluorescence to detect DORs, SEB was found to increase the fraction of T cells that expressed DOR and to enhance the relative level of DOR expression per T cell. Previous studies have shown that DOR agonists inhibited the anti-CD3-stimulated production of interleukin-2 and T-cell proliferation. Therefore, the enhanced expression of DORs by activated T cells may be capable of downregulating the T-cell activation program.

UR - http://www.scopus.com/inward/record.url?scp=0033671181&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033671181&partnerID=8YFLogxK

M3 - Article

C2 - 11268405

AN - SCOPUS:0033671181

VL - 917

SP - 764

EP - 770

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

SN - 0077-8923

ER -