Expression of delta opioid receptors by splenocytes from SEB-treated mice and effects on phosphorylation of MAP kinase

Nahid A. Shahabi, Kathy McAllen, Shannon G. Matta, Burt Sharp

Research output: Contribution to journalArticle

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Abstract

Delta opioid receptors (DORs) are known to modulate multiple T-Cell responses. However, little is known about the expression of these receptors. These studies evaluated the expression of DOR mRNA and protein after a single in vivo exposure to staphylococcal enterotoxin B (SEB). SEB (20 μg, ip) significantly enhanced splenocyte DOR mRHA expression 8 and 24 h after injection. SEB also increased the fractions of tile total splenocyte (5 to 20%) and T-Cell (8 to 50%) populations expressing DOR protein. In saline-treated animals, DOR relative fluorescence intensity per cell was 11.1 ± 0.62 units (mean ± SEM), increasing to 16.1 ± 1.7 after exposure to SEB. DOR fluorescence intensity significantly increased to 33.5 ± 2.6 units in a subpopulation of T-cells. Thus, SEB significantly increased DOR expression in vivo, affecting both mRNA and protein levels primarily within the T-cell population. To determine whether T-cell DORs modulate the activity of extracellular-regulated kinases (ERKs), the phosphorylation of ERKs I and 2 was studied using splenocytes from SEB-Treated mice. At concentrations from 10-8 to 10-6 M, [D-Ala2-D-Leu5]-Enkephalin, a selective DOR agonist, significantly inhibited anti-CD3-<nf>-induced phosphorylation of the ERKs. Therefore, the DORs expressed by activated T-cells are capable of attenuating T-cell activation that depends on ERK phosphorylation. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)84-93
Number of pages10
JournalCellular Immunology
Volume205
Issue number2
DOIs
StatePublished - Nov 1 2000

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delta Opioid Receptor
Phosphotransferases
Phosphorylation
T-Lymphocytes
Fluorescence
staphylococcal enterotoxin B
Messenger RNA
Proteins
Population

All Science Journal Classification (ASJC) codes

  • Immunology

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Expression of delta opioid receptors by splenocytes from SEB-treated mice and effects on phosphorylation of MAP kinase. / Shahabi, Nahid A.; McAllen, Kathy; Matta, Shannon G.; Sharp, Burt.

In: Cellular Immunology, Vol. 205, No. 2, 01.11.2000, p. 84-93.

Research output: Contribution to journalArticle

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