Expression of Egr-1 in late stage emphysema

Weisu Zhang, Shi Du Yan, Aiping Zhu, Yu Shan Yu Shan Zou, Matthew Williams, Gabriel C. Godman, Byron M. Thomashow, Mark E. Ginsburg, David Stern, Shi Fang Yan

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The transcription factor early growth response (Egr)-1 is an immediate-early gene product rapidly and transiently expressed after acute tissue injury. In contrast, in this report we demonstrate that lung tissue from patients undergoing lung reduction surgery for advanced emphysema, without clinical or anatomical evidence of acute infection, displays a selective and apparently sustained increase in Egr-1 transcripts and antigen, compared with a broad survey of other genes, including the transcription factor Sp1, whose levels were not significantly altered. Enhanced Egr-1 expression was especially evident in smooth muscle cells of bronchial and vascular walls, in alveolar macrophages, and some vascular endothelium. Gel shift analysis with 32 P-labeled Egr probe showed a band with nuclear extracts from emphysematous lung which was supershifted with antibody to Egr-1. Egr-1 has the capacity to regulate genes relevant to the pathophysiology of emphysema, namely those related to extracellular matrix formation and remodeling, thrombogenesis, and those encoding cytokines/chemokines and growth factors. Thus, we propose that further analysis of Egr-1, which appears to be up-regulated in a sustained fashion in patients with late stage emphysema, may provide insights into the pathogenesis of this destructive pulmonary disease, as well as a new facet in the biology of Egr-1.

Original languageEnglish (US)
Pages (from-to)1311-1320
Number of pages10
JournalAmerican Journal of Pathology
Volume157
Issue number4
DOIs
StatePublished - Jan 1 2000

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Emphysema
Growth
Lung
Early Growth Response Protein 1
Sp1 Transcription Factor
Immediate-Early Genes
Vascular Endothelium
Alveolar Macrophages
Electrophoretic Mobility Shift Assay
Chemokines
Lung Diseases
Genes
Smooth Muscle Myocytes
Extracellular Matrix
Blood Vessels
Intercellular Signaling Peptides and Proteins
Cytokines
Antigens
Antibodies
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Zhang, W., Yan, S. D., Zhu, A., Yu Shan Zou, Y. S., Williams, M., Godman, G. C., ... Yan, S. F. (2000). Expression of Egr-1 in late stage emphysema. American Journal of Pathology, 157(4), 1311-1320. https://doi.org/10.1016/S0002-9440(10)64646-9

Expression of Egr-1 in late stage emphysema. / Zhang, Weisu; Yan, Shi Du; Zhu, Aiping; Yu Shan Zou, Yu Shan; Williams, Matthew; Godman, Gabriel C.; Thomashow, Byron M.; Ginsburg, Mark E.; Stern, David; Yan, Shi Fang.

In: American Journal of Pathology, Vol. 157, No. 4, 01.01.2000, p. 1311-1320.

Research output: Contribution to journalArticle

Zhang, W, Yan, SD, Zhu, A, Yu Shan Zou, YS, Williams, M, Godman, GC, Thomashow, BM, Ginsburg, ME, Stern, D & Yan, SF 2000, 'Expression of Egr-1 in late stage emphysema', American Journal of Pathology, vol. 157, no. 4, pp. 1311-1320. https://doi.org/10.1016/S0002-9440(10)64646-9
Zhang W, Yan SD, Zhu A, Yu Shan Zou YS, Williams M, Godman GC et al. Expression of Egr-1 in late stage emphysema. American Journal of Pathology. 2000 Jan 1;157(4):1311-1320. https://doi.org/10.1016/S0002-9440(10)64646-9
Zhang, Weisu ; Yan, Shi Du ; Zhu, Aiping ; Yu Shan Zou, Yu Shan ; Williams, Matthew ; Godman, Gabriel C. ; Thomashow, Byron M. ; Ginsburg, Mark E. ; Stern, David ; Yan, Shi Fang. / Expression of Egr-1 in late stage emphysema. In: American Journal of Pathology. 2000 ; Vol. 157, No. 4. pp. 1311-1320.
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