Expression of human eNOS in cardiac and endothelial cells.

Xiuqing Wang, Robert Cade, Zhongjie Sun

Research output: Contribution to journalReview article

10 Citations (Scopus)

Abstract

In this chapter we provide a detailed technique-focused protocol for expression of human endothelial nitric oxide synthase (eNOS) gene delivered by replication-deficient adenovirus. It includes construction of recombinant plasmid with human eNOS gene full-length cDNA (heNOS), packaging of adenovirus with heNOS (Adv-heNOS) in 293 cells, confirmation of Adv-heNOS, amplification of high-titer stockAdv-heNOS, determination of the Adv-heNOS titer, expression of Adv-heNOS in the human abdominal aorta endothelial (HAAE1) cell line and rat heart myoblast cell line (H9C2), and measurement of human eNOS activity and NO production.

Original languageEnglish (US)
Pages (from-to)91-107
Number of pages17
JournalMethods in molecular medicine
Volume112
StatePublished - Jan 1 2005
Externally publishedYes

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Nitric Oxide Synthase Type III
Adenoviridae
Endothelial Cells
Cell Line
Myoblasts
Abdominal Aorta
Product Packaging
Genes
Plasmids
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Molecular Medicine

Cite this

Expression of human eNOS in cardiac and endothelial cells. / Wang, Xiuqing; Cade, Robert; Sun, Zhongjie.

In: Methods in molecular medicine, Vol. 112, 01.01.2005, p. 91-107.

Research output: Contribution to journalReview article

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