Expression of KIR and C-type lectin receptors in Behçet's disease

G. Saruhan-Direskeneli, F. A. Uyar, A. Çefle, Songul Onder, E. Ekşioǧlu-Demiralp, S. Kamali, M. Inanç, L. Ocal, A. Gül

Research output: Contribution to journalArticle

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Abstract

Objective. Behçet's disease (BD) is a multisystemic disorder with a possible underlying pathology of immune-mediated vasculitis. Genetic susceptibility associated with HLA-B*51 and B*2702 has been implicated in its pathogenesis. Considering the recently defined regulatory mechanisms of NK cells through HLA class I binding receptors, we hypothesized that interactions of NK and T cells through the NK receptors may be important in the pathogenesis of BD. Methods. The impact of different expression patterns of HLA-recognizing receptors on NK or T cells was analysed in 51 patients with BD and 32 healthy controls. We used flow cytometry to investigate the expression of KIR3DL1 from the polymorphic killer immunoglobulin-like receptor (KIR) family, which binds a shared HLA-Bw4 motif on HLA-B51 and *2702 alleles, and CD94 from the conserved C-type lectin receptor family, which binds HLA-E. Thirty-three of the BD patients and 19 of the controls carried the same HLA-Bw4 motif. Results. CD3+ T cells were increased in patients with BD compared with controls (81 vs 75%, P = 0.001), whereas the NK cells did not show any difference between the two groups. Increased expression of CD94 in BD was observed on CD16+CD56+ cells (66 vs 57, P = 0.04) and on CD3+ (7.7 vs 4.0, P < 0.001) and CD3+CD56+ (44 vs 35, P = 0.02) T cells. KIR3DL1 expression on the NK and T cells was not statistically different between the two groups. No effect of HLA-Bw4 motif was observed on the expression of CD94 and KIR3DL1 in both the patients and the controls. Conclusion. The absence of a correlation between KIR3DL1 expression and HLA-Bw4 motif confirms previous work reporting that the expression of these molecules is regulated separately. Increased expression of CD94 may suggest that NK receptors play a pathogenic or regulatory role in BD.

Original languageEnglish (US)
Pages (from-to)423-427
Number of pages5
JournalRheumatology
Volume43
Issue number4
DOIs
StatePublished - Apr 1 2004

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KIR Receptors
C-Type Lectins
Natural Killer Cells
T-Lymphocytes
HLA-B51 Antigen
Natural Killer Cell Receptors
HLA-B Antigens
Genetic Predisposition to Disease
Vasculitis
T-Cell Antigen Receptor
Flow Cytometry
Alleles
Pathology
HLA-Bw4 antigen

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Pharmacology (medical)

Cite this

Saruhan-Direskeneli, G., Uyar, F. A., Çefle, A., Onder, S., Ekşioǧlu-Demiralp, E., Kamali, S., ... Gül, A. (2004). Expression of KIR and C-type lectin receptors in Behçet's disease. Rheumatology, 43(4), 423-427. https://doi.org/10.1093/rheumatology/keh063

Expression of KIR and C-type lectin receptors in Behçet's disease. / Saruhan-Direskeneli, G.; Uyar, F. A.; Çefle, A.; Onder, Songul; Ekşioǧlu-Demiralp, E.; Kamali, S.; Inanç, M.; Ocal, L.; Gül, A.

In: Rheumatology, Vol. 43, No. 4, 01.04.2004, p. 423-427.

Research output: Contribution to journalArticle

Saruhan-Direskeneli, G, Uyar, FA, Çefle, A, Onder, S, Ekşioǧlu-Demiralp, E, Kamali, S, Inanç, M, Ocal, L & Gül, A 2004, 'Expression of KIR and C-type lectin receptors in Behçet's disease', Rheumatology, vol. 43, no. 4, pp. 423-427. https://doi.org/10.1093/rheumatology/keh063
Saruhan-Direskeneli G, Uyar FA, Çefle A, Onder S, Ekşioǧlu-Demiralp E, Kamali S et al. Expression of KIR and C-type lectin receptors in Behçet's disease. Rheumatology. 2004 Apr 1;43(4):423-427. https://doi.org/10.1093/rheumatology/keh063
Saruhan-Direskeneli, G. ; Uyar, F. A. ; Çefle, A. ; Onder, Songul ; Ekşioǧlu-Demiralp, E. ; Kamali, S. ; Inanç, M. ; Ocal, L. ; Gül, A. / Expression of KIR and C-type lectin receptors in Behçet's disease. In: Rheumatology. 2004 ; Vol. 43, No. 4. pp. 423-427.
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AU - Uyar, F. A.

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AU - Onder, Songul

AU - Ekşioǧlu-Demiralp, E.

AU - Kamali, S.

AU - Inanç, M.

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