Expression of leptin and β3-adrenergic receptors in rat adipose tissue in altered thyroid states

John N. Fain, Elizabeth C. Coronel, Michael J. Beauchamp, Suleiman Bahouth

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The level of leptin [the obese (ob) gene product] mRNA is markedly elevated in hypothyroid male rats. The administration of tri-iodothyronine (T3) to hypothyroid rats resulted in a 40% decrease in leptin mRNA at 8 h. This decrease in leptin mRNA was associated with a parallel decline in circulating leptin levels of about 50% at 24 h. Conversely, β2-adrenergic receptor mRNA levels were markedly decreased in epididymal adipose tissue from hypothyroid rats. T3 administration resulted in a 147% increase at 12 h in β2-adrenergic receptor mRNA. There was a corresponding increase due to T3 in the lipolytic response to the specific β3-adrenergic agonist CL 316,243 that paralleled the increase in β3-adrenergic receptor mRNA. T3-mediated changes in leptin and β2-adrenergic receptor mRNAs were blocked by cycloheximide, suggesting the involvement of short-lived proteins in these effects. The present results indicate that T3 has opposite effects to those of insulin on the white adipose tissue of rats with respect to leptin mRNA expression.

Original languageEnglish (US)
Pages (from-to)145-150
Number of pages6
JournalBiochemical Journal
Volume322
Issue number1
DOIs
StatePublished - Feb 15 1997

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Leptin
Adrenergic Receptors
Adipose Tissue
Rats
Thyroid Gland
Tissue
Messenger RNA
White Adipose Tissue
Adrenergic Agonists
Cycloheximide
Insulin

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Expression of leptin and β3-adrenergic receptors in rat adipose tissue in altered thyroid states. / Fain, John N.; Coronel, Elizabeth C.; Beauchamp, Michael J.; Bahouth, Suleiman.

In: Biochemical Journal, Vol. 322, No. 1, 15.02.1997, p. 145-150.

Research output: Contribution to journalArticle

Fain, John N. ; Coronel, Elizabeth C. ; Beauchamp, Michael J. ; Bahouth, Suleiman. / Expression of leptin and β3-adrenergic receptors in rat adipose tissue in altered thyroid states. In: Biochemical Journal. 1997 ; Vol. 322, No. 1. pp. 145-150.
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