Expression of the monocytic differentiation/activation factor P48 in Mycoplasma species

Robert E. Hall, Daniel Kestler, Sujata Agarwal, Keith M. Goldstein

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

P48 is a 48 kd monocytic differentiation/activation factor previously purified from the conditioned medium of the Reh human pre-B cell leukemia line. It induces differentiation of HL-60 promyelocytic leukemia cells along the monocytic pathway and production of IL1, TNF-α and IL6 in human monocytes and monocytic cell lines. Recently our laboratory isolated cDNA clones for P48 from Reh cells and genomic clones from Mycoplasma fermentans DNA and showed that P48 is a M. fermentans gene product. In this paper we report the analysis of P48 expression at the DNA, mRNA and protein levels in different Mycoplasma species. Polymerase Chain Reaction (PCR) analysis of extracted DNA using P48-specific oligonucleotide primers revealed P48 sequences in M. fermentans but not M. hominis, M. iowae, M. genitalium or M. capricolum. Southern analysis of Mycoplasma DNAs revealed hybridizing bands in M. fermentans and M. capricolum under low stringency, but only in M. fermentans under high stringency. Consistent with this, Northern blot studies revealed a single hybridizing transcript in M. fermentans but not in other Mycoplasma species tested. However, Western blot studies with anti-P48 antibodies revealed P48 antigenic material in M. fermentans, as well as M. hominis and M. iowae. These studies demonstrate that the gene for P48 is derived from M. fermentans or a closely related species and is absent in these other species tested. However, the P48 protein exhibits shared antigenic determinants among several Mycoplasma species which presently are of unknown function or significance. P48 is a Mycoplasma-derived immunomodulatory molecule which may be important in Mycoplasma pathophysiology and may be useful in understanding human haematopoietic differentiation and the control of cytokine biosynthesis.

Original languageEnglish (US)
Pages (from-to)145-153
Number of pages9
JournalMicrobial Pathogenesis
Volume27
Issue number3
DOIs
StatePublished - Jan 1 1999

Fingerprint

Mycoplasma fermentans
Mycoplasma
DNA
Clone Cells
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma
Cell Line
DNA Primers
monocytic differentiation factor P48
Conditioned Culture Medium
Northern Blotting
Genes
Epitopes
Monocytes
Anti-Idiotypic Antibodies
Interleukin-6
Leukemia
Proteins
Complementary DNA
Western Blotting
Cytokines

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Infectious Diseases

Cite this

Expression of the monocytic differentiation/activation factor P48 in Mycoplasma species. / Hall, Robert E.; Kestler, Daniel; Agarwal, Sujata; Goldstein, Keith M.

In: Microbial Pathogenesis, Vol. 27, No. 3, 01.01.1999, p. 145-153.

Research output: Contribution to journalArticle

Hall, Robert E. ; Kestler, Daniel ; Agarwal, Sujata ; Goldstein, Keith M. / Expression of the monocytic differentiation/activation factor P48 in Mycoplasma species. In: Microbial Pathogenesis. 1999 ; Vol. 27, No. 3. pp. 145-153.
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