Expression of the multifunctional Y-box protein, YB-1, in myofibroblasts of the infarcted rat heart

German Kamalov, Balwantkumar R. Varma, Li Lu, Yao Sun, Karl Weber, Ramareddy Guntaka

Research output: Contribution to journalArticle

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Abstract

Intracellular signaling mechanisms regulating the turnover of α-SMA-positive myofibroblasts (myoFbs) at the site of myocardial infarction (MI) are poorly understood. Y-Box (YB)-1, a multifunctional protein, may be involved in regulation of proliferation, migration and apoptosis of myoFbs. Our objective was to study the expression of YB-1 in the infarcted rat heart and its localization in myoFbs. On days 3-28 following MI, we monitored YB-1 expression and its colocalization with α-SMA, and proliferation markers PCNA and Ki-67 in infarcted tissue by Western blot, immunohistochemistry, and immunofluorescent double-labeling. YB-1 is barely detectable in normal myocardium. At the infarct site, however, YB-1 is markedly elevated from day 3 post-MI concomitant with the induction of cell proliferation. MyoFbs are the major source of YB-1 and retain it up to day 28 post-MI. We suggest early expression of YB-1 promotes proliferation and migration of myoFbs, whereas prolonged expression may be responsible for scar formation.

Original languageEnglish (US)
Pages (from-to)239-244
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume334
Issue number1
DOIs
StatePublished - Aug 19 2005

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Y-Box-Binding Protein 1
Myofibroblasts
Proliferating Cell Nuclear Antigen
Cell proliferation
Labeling
Rats
Myocardial Infarction
Tissue
Apoptosis
Proteins
Cicatrix
Myocardium
Western Blotting
Immunohistochemistry
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Expression of the multifunctional Y-box protein, YB-1, in myofibroblasts of the infarcted rat heart. / Kamalov, German; Varma, Balwantkumar R.; Lu, Li; Sun, Yao; Weber, Karl; Guntaka, Ramareddy.

In: Biochemical and Biophysical Research Communications, Vol. 334, No. 1, 19.08.2005, p. 239-244.

Research output: Contribution to journalArticle

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AU - Weber, Karl

AU - Guntaka, Ramareddy

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