Expression of the rat liver carnitine palmitoyltransferase I (CPT-Iα) gene is regulated by Sp1 and nuclear factor Y

Chromosomal localization and promoter characterization

Michelle L. Steffen, Wilbur R. Harrison, Frederick F B Elder, George Cook, Edwards Park

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Carnitine palmitoyltransferase (CPT)-I catalyses the transfer of long-chain fatty acids from CoA to carnitine for translocation across the mitochondrial inner membrane. Expression of the 'liver' isoform of the CPT-I gene (CPT-Iα) is subject to developmental, hormonal and tissue-specific regulation. To understand the basis for control of CPT-Iα gene expression, we have characterized the proximal promoter of the CPT-Iα gene. Here, we report the sequence of 6839 base pairs of the promoter and the localization of the rat CPT-Iα gene to region q43 on chromosome 1. Our studies show that the first 200 base pairs of the promoter are sufficient to drive transcription of the CPT-Iα gene. Within this region are two sites that bind both Sp1 and Sp3 transcription factors. In addition, nuclear factor Y (NF-Y) binds the proximal promoter. Mutation at the Sp1 or NF-Y sites severely decreases transcription from the CPT-Iα promoter. Other protein binding sites were identified within the first 200 base pairs of the promoter by DNase I footprinting, and these elements contribute to CPT-Iα gene expression. Our studies demonstrate that CPT-Iα is a TATA-less gene which utilizes NF-Y and Sp proteins to drive basal expression.

Original languageEnglish (US)
Pages (from-to)425-432
Number of pages8
JournalBiochemical Journal
Volume340
Issue number2
DOIs
StatePublished - Jun 1 1999

Fingerprint

Carnitine O-Palmitoyltransferase
Liver
Rats
Genes
Base Pairing
Transcription
Gene expression
Sp3 Transcription Factor
nuclear factor Y
Sp1 Transcription Factor
Gene Expression
Carnitine
Chromosomes, Human, Pair 1
Deoxyribonuclease I
Mitochondrial Membranes
Coenzyme A
Chromosomes
Protein Binding
Protein Isoforms
Fatty Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Expression of the rat liver carnitine palmitoyltransferase I (CPT-Iα) gene is regulated by Sp1 and nuclear factor Y : Chromosomal localization and promoter characterization. / Steffen, Michelle L.; Harrison, Wilbur R.; Elder, Frederick F B; Cook, George; Park, Edwards.

In: Biochemical Journal, Vol. 340, No. 2, 01.06.1999, p. 425-432.

Research output: Contribution to journalArticle

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