Expression of transforming growth factor-beta 1, -beta 2, and -beta 3 mRNA and protein in the murine lung.

R. W. Pelton, Mahlon Johnson, E. A. Perkett, L. I. Gold, H. L. Moses

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Evidence has accumulated suggesting that the various isoforms of beta-type transforming growth factors (TGF-beta s) regulate important functions in the lung; however, the cellular source of these proteins is not well defined. Northern blot analysis of murine lung tissue demonstrates that mRNA transcripts for all three TGF-beta isoforms are found from birth through adulthood. Although the level of expression for each TGF-beta is variable during the first 2 wk post partum, all three isoforms are equal in the adult lung. Using in situ hybridization and immunohistochemical analysis, we have localized both mRNA and protein expression for all three isoforms of TGF-beta in the adult murine lung. At low magnification, immunohistochemical localization of TGF-beta proteins appears coincident in their pattern of expression with TGF-beta mRNAs in the large proximal conducting airways of the lung. However, on closer analysis, protein expression of all three TGF-beta isoforms is confined to the bronchiolar epithelium, while TGF-beta mRNA transcripts for each of the TGF-beta genes are found in smooth muscle cells and connective tissue fibroblasts lying subjacent to the epithelium. Although the levels of both TGF-beta mRNA and protein expression are high in the proximal bronchiolar tree, their signal intensities completely disappear as the terminal bronchioles progress to respiratory bronchioles. Additionally, in the lung vasculature, there is very high expression of all three TGF-beta mRNA transcripts in the smooth muscle cells of the large vessels. TGF-beta2 and TGF-beta but not TGF-beta1 proteins are expressed in these same smooth muscle cells.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)522-530
Number of pages9
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume5
Issue number6
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

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Transforming Growth Factor beta
Lung
Messenger RNA
Proteins
Protein Isoforms
Smooth Muscle Myocytes
Bronchioles
Muscle
Cells
Epithelium
Transforming Growth Factor beta2
Tissue
Transforming Growth Factor beta1
Fibroblasts
Northern Blotting
Connective Tissue
In Situ Hybridization
Genes
Parturition
Muscles

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Expression of transforming growth factor-beta 1, -beta 2, and -beta 3 mRNA and protein in the murine lung. / Pelton, R. W.; Johnson, Mahlon; Perkett, E. A.; Gold, L. I.; Moses, H. L.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 5, No. 6, 01.01.1991, p. 522-530.

Research output: Contribution to journalArticle

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