Expression pattern of entire cytochrome P450 genes and response of defensomes in the benzo[a]pyrene-exposed monogonont rotifer brachionus koreanus

Ryeo Ok Kim, Bo Mi Kim, Chang Bum Jeong, David Nelson, Jae Seong Lee, Jae Sung Rhee

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Cytochrome P450 (CYP) proteins are involved in the first line of detoxification mechanism against diverse polycyclic aromatic hydrocarbons (PAHs) including benzo[a]pyrene (B[a]P). In aquatic invertebrates, there is still a lack of knowledge on the CYP genes involved in the molecular response to B[a]P exposure due to limited gene information. In this study, we cloned the entire 25 CYP genes in the monogonont rotifer Brachionus koreanus with the aid of next generation sequencing (NGS) technologies and analyzed their transcript profiles with a real-time RT-PCR array to better understand B[a]P-triggered molecular response over different time courses. As a result, B[a]P exposure induced CYP2/3-involved detoxification mechanisms and defensome, including phase II detoxification and antioxidant systems with a modulation of the chaperone heat shock protein (hsp) expression but did not change expression of other CYP clans in B. koreanus. Therefore, we found that B[a]P induced a strong detoxification mechanism to overcome detrimental effects of B[a]P associated with B[a]P-induced growth retardation as a trade-off in fitness costs. Also, this approach revealed that the entire CYP profiling can be a way of providing a better understanding on the mode of action of B[a]P in B. koreanus with respect to molecular defense metabolism.

Original languageEnglish (US)
Pages (from-to)13804-13812
Number of pages9
JournalEnvironmental Science and Technology
Volume47
Issue number23
DOIs
StatePublished - Dec 3 2013

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Benzo(a)pyrene
pyrene
Cytochrome P-450 Enzyme System
cytochrome
Genes
Detoxification
gene
detoxification
protein
Polycyclic Aromatic Hydrocarbons
heat shock
Heat-Shock Proteins
Metabolism
antioxidant
trade-off
PAH
fitness
Antioxidants
invertebrate
metabolism

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Expression pattern of entire cytochrome P450 genes and response of defensomes in the benzo[a]pyrene-exposed monogonont rotifer brachionus koreanus. / Kim, Ryeo Ok; Kim, Bo Mi; Jeong, Chang Bum; Nelson, David; Lee, Jae Seong; Rhee, Jae Sung.

In: Environmental Science and Technology, Vol. 47, No. 23, 03.12.2013, p. 13804-13812.

Research output: Contribution to journalArticle

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