Expression pattern of messenger RNAs for prostanoid receptors in glial cell cultures

Junichi Kitanaka, Hitoshi Hashimoto, Masayuki Gotoh, Kaoru Kondo, Kazuko Sakata, Yoshimi Hirasawa, Makoto Sawada, Akio Suzumura, Tohru Marunouchi, Toshio Matsuda, Akemichi Baba

Research output: Contribution to journalArticle

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Abstract

Expression level of messenger RNAs (mRNAs) for prostanoid EP3, FP, and TP receptors was investigated in cultured rat astrocytes, oligodendrocytes, and microglia, as well as in meningeal fibroblasts, rat glioma C6 cells, rat pheochromocytoma PC12 cells, whole brain, and several peripheral tissues by reverse transcriptase-polymerase chain reaction. Cultured astrocytes and oligodendrocytes expressed mRNAs for 3 prostanoid receptors examined. In contrast, cultured microglia and pheochromocytoma PC12 cells expressed EP3 and TP receptor mRNAs, but not FP receptor mRNA. Glioma C6 cells expressed only TP receptor mRNA among 3 prostanoid receptors with the same expression level as that in astrocytes. Cultured meningeal fibroblasts expressed 3 receptor transcripts, and their expression levels were lower than those in astrocytes. Expression level of mRNA for each prostanoid receptor in cultured glial cells was higher than that in whole brain. These observations suggest that each prostanoid has its specific roles in each glial cell type of the brain.

Original languageEnglish (US)
Pages (from-to)282-287
Number of pages6
JournalBrain Research
Volume707
Issue number2
DOIs
StatePublished - Jan 29 1996

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Neuroglia
Prostaglandins
Cell Culture Techniques
Thromboxane Receptors
Astrocytes
Messenger RNA
PC12 Cells
Oligodendroglia
Microglia
Pheochromocytoma
Glioma
Brain
Fibroblasts
Reverse Transcriptase Polymerase Chain Reaction
Cultured Cells
prostaglandin F2alpha receptor

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Kitanaka, J., Hashimoto, H., Gotoh, M., Kondo, K., Sakata, K., Hirasawa, Y., ... Baba, A. (1996). Expression pattern of messenger RNAs for prostanoid receptors in glial cell cultures. Brain Research, 707(2), 282-287. https://doi.org/10.1016/0006-8993(95)01256-7

Expression pattern of messenger RNAs for prostanoid receptors in glial cell cultures. / Kitanaka, Junichi; Hashimoto, Hitoshi; Gotoh, Masayuki; Kondo, Kaoru; Sakata, Kazuko; Hirasawa, Yoshimi; Sawada, Makoto; Suzumura, Akio; Marunouchi, Tohru; Matsuda, Toshio; Baba, Akemichi.

In: Brain Research, Vol. 707, No. 2, 29.01.1996, p. 282-287.

Research output: Contribution to journalArticle

Kitanaka, J, Hashimoto, H, Gotoh, M, Kondo, K, Sakata, K, Hirasawa, Y, Sawada, M, Suzumura, A, Marunouchi, T, Matsuda, T & Baba, A 1996, 'Expression pattern of messenger RNAs for prostanoid receptors in glial cell cultures', Brain Research, vol. 707, no. 2, pp. 282-287. https://doi.org/10.1016/0006-8993(95)01256-7
Kitanaka, Junichi ; Hashimoto, Hitoshi ; Gotoh, Masayuki ; Kondo, Kaoru ; Sakata, Kazuko ; Hirasawa, Yoshimi ; Sawada, Makoto ; Suzumura, Akio ; Marunouchi, Tohru ; Matsuda, Toshio ; Baba, Akemichi. / Expression pattern of messenger RNAs for prostanoid receptors in glial cell cultures. In: Brain Research. 1996 ; Vol. 707, No. 2. pp. 282-287.
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