Expression patterns of organic anion transporting polypeptides 1B1 and 1B3 protein in human pediatric liver

Margaret M.S. Thomson, Ronald N. Hines, Erin G. Schuetz, Bernd Meibohm

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Determining appropriate pharmacotherapy in young children can be challenging due to uncertainties in the development of drug disposition pathways. With knowledge of the ontogeny of drug-metabolizing enzymes and an emerging focus on drug transporters, the developmental pattern of the uptake transporters organic anion transporting polypeptide (OATP) 1B1 and 1B3 was assessed by relative protein quantification using Western blotting in 80 human pediatric liver specimens covering an age range from 9 days to 12 years. OATP1B3 exhibited high expression at birth, which declined over the first months of life, and then increased again in the preadolescent period. In comparison with children 6-12 years of age, the relative protein expression of highly glycosylated (total) OATP1B3 was 235% (357%) in children <3 months of age, 33% (64%) in the age group from 3 months to 2 years, and 50% (59%) in children 2-6 years of age. The fraction of highly glycosylated to total OATP1B3 increased with age, indicating ontogenic processes not only at the transcriptional level but also at the post-translational level. Similar to OATP1B3, OATP1B1 showed high interindividual variability in relative protein expression but no statistically significant difference among the studied age groups.

Original languageEnglish (US)
Pages (from-to)999-1004
Number of pages6
JournalDrug Metabolism and Disposition
Volume44
Issue number7
DOIs
StatePublished - Jul 1 2016

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Anions
Pediatrics
Peptides
Liver
Proteins
Age Groups
Organic Anion Transporters
Pharmaceutical Preparations
Uncertainty
Western Blotting
Parturition
Drug Therapy
Enzymes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Expression patterns of organic anion transporting polypeptides 1B1 and 1B3 protein in human pediatric liver. / Thomson, Margaret M.S.; Hines, Ronald N.; Schuetz, Erin G.; Meibohm, Bernd.

In: Drug Metabolism and Disposition, Vol. 44, No. 7, 01.07.2016, p. 999-1004.

Research output: Contribution to journalArticle

Thomson, Margaret M.S. ; Hines, Ronald N. ; Schuetz, Erin G. ; Meibohm, Bernd. / Expression patterns of organic anion transporting polypeptides 1B1 and 1B3 protein in human pediatric liver. In: Drug Metabolism and Disposition. 2016 ; Vol. 44, No. 7. pp. 999-1004.
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