Expression quantitative trait loci and genetic regulatory network analysis reveals that Gabra2 is involved in stress responses in the mouse

Jiajuan Dai, Xusheng Wang, Ying Chen, Xiaodong Wang, Jun Zhu, Lu Lu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Previous studies have revealed that the subunit α 2 (Gabra2) of the γ-aminobutyric acid receptor plays a critical role in the stress response. However, little is known about the gentetic regulatory network for Gabra2 and the stress response. We combined gene expression microarray analysis and quantitative trait loci (QTL) mapping to characterize the genetic regulatory network for Gabra2 expression in the hippocampus of BXD recombinant inbred (RI) mice. Our analysis found that the expression level of Gabra2 exhibited much variation in the hippocampus across the BXD RI strains and between the parental strains, C57BL/6J, and DBA/2J. Expression QTL (eQTL) mapping showed three microarray probe sets of Gabra2 to have highly significant linkage likelihood ratio statistic (LRS) scores. Gene co-regulatory network analysis showed that 10 genes, including Gria3, Chka, Drd3, Homer1, Grik2, Odz4, Prkag2, Grm5, Gabrb1, and Nlgn1 are directly or indirectly associated with stress responses. Eleven genes were implicated as Gabra2 downstream genes through mapping joint modulation. The genetical genomics approach demonstrates the importance and the potential power of the eQTL studies in identifying genetic regulatory networks that contribute to complex traits, such as stress responses.

Original languageEnglish (US)
Pages (from-to)499-506
Number of pages8
JournalStress
Volume12
Issue number6
DOIs
StatePublished - Jan 1 2009

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Quantitative Trait Loci
Hippocampus
Gene Regulatory Networks
Chromosome Mapping
Microarray Analysis
Genomics
Genes
Joints
Gene Expression
alpha-aminobutyric acid

All Science Journal Classification (ASJC) codes

  • Physiology
  • Neuropsychology and Physiological Psychology
  • Endocrine and Autonomic Systems
  • Psychiatry and Mental health
  • Behavioral Neuroscience

Cite this

Expression quantitative trait loci and genetic regulatory network analysis reveals that Gabra2 is involved in stress responses in the mouse. / Dai, Jiajuan; Wang, Xusheng; Chen, Ying; Wang, Xiaodong; Zhu, Jun; Lu, Lu.

In: Stress, Vol. 12, No. 6, 01.01.2009, p. 499-506.

Research output: Contribution to journalArticle

Dai, Jiajuan ; Wang, Xusheng ; Chen, Ying ; Wang, Xiaodong ; Zhu, Jun ; Lu, Lu. / Expression quantitative trait loci and genetic regulatory network analysis reveals that Gabra2 is involved in stress responses in the mouse. In: Stress. 2009 ; Vol. 12, No. 6. pp. 499-506.
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