Extracellular matrix-dependent regulation of angiogenin expression in human placenta

Raja Shekhar Gangaraju, Annamalai Loganath, A. C. Roy, S. S. Chong, Y. C. Wong

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Knowledge of the rapidly developing hierarchy of controls affecting vascular development in placenta is required to understand how the growth factors and their receptor-mediated signals actually produce vessels. At the cell biological level, these events clearly require stable interactions between the cells, and cells with the surrounding ECM. The objective of the study was to understand the role of integrins and ECM on the expression and secretion of angiogenin in placentas and from trophoblasts in culture. Functionally active term placental explant culture and trophoblast cultures were used to demonstrate the differential secretion profile of angiogenin and real-time quantitative RT-PCR to demonstrate the mRNA expression in the presence or absence of ECM proteins. In this study, a significant increase in expression and secretion of angiogenin occurred in the presence of vitronectin (VN) and fibronectin (FN). Using antibody-blocking experiments it was also demonstrated that the angiogenin secretion is mediated by placental integrins, α v β 3 and α 5 β1. In addition, exposure to hypoxic conditions resulted in diminished angiogenin secretion in the presence of both ECMs suggesting that angiogenin expression in the presence of ECM is modulated by local O 2 concentration. In conclusion, this study provides evidence for the regulatory role of ECM and integrins on the mRNA expression and secretion of angiogenin in human placenta. ECMs may have a pivotal role in enhancing secretion of this peptide necessary for placental angiogenesis and provides the impetus as additional targets for the control of angiogenesis in pathological pregnancy.

Original languageEnglish (US)
Pages (from-to)36-46
Number of pages11
JournalJournal of Cellular Biochemistry
Volume96
Issue number1
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

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Military electronic countermeasures
Placenta
Extracellular Matrix
Integrins
Trophoblasts
Pathologic Neovascularization
Vitronectin
Messenger RNA
Blocking Antibodies
Growth Factor Receptors
angiogenin
Fibronectins
Cell Communication
Blood Vessels
Real-Time Polymerase Chain Reaction
Pregnancy
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Extracellular matrix-dependent regulation of angiogenin expression in human placenta. / Gangaraju, Raja Shekhar; Loganath, Annamalai; Roy, A. C.; Chong, S. S.; Wong, Y. C.

In: Journal of Cellular Biochemistry, Vol. 96, No. 1, 01.09.2005, p. 36-46.

Research output: Contribution to journalArticle

Gangaraju, Raja Shekhar ; Loganath, Annamalai ; Roy, A. C. ; Chong, S. S. ; Wong, Y. C. / Extracellular matrix-dependent regulation of angiogenin expression in human placenta. In: Journal of Cellular Biochemistry. 2005 ; Vol. 96, No. 1. pp. 36-46.
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