Extraction of 3,4,4'-trichlorocarbanilide from rat fecal samples for determination by high pressure liquid chromatography–tandem mass spectrometry

Rebekah C. Kennedy, Russell R. Fling, Paul D. Terry, Fu Min Menn, Jiangang Chen, Christopher J. Borman

Research output: Contribution to journalArticle

Abstract

Triclocarban (3,4,4'-Trichlorocarbanilide; TCC) in the environment has been well documented. Methods have been developed to monitor TCC levels from various matrices including water, sediment, biosolids, plants, blood and urine; however, no method has been developed to document the concentration of TCC in fecal content after oral exposure in animal studies. In the present study, we developed and validated a method that uses liquid extraction coupled with HPLC-MS/MS determination to measure TCC in feces. The limit of detection and limit of quantitation in control rats without TCC exposure was 69.0 ng/g and 92.9 ng/g of feces, respectively. The base levels of TCC in feces were lower than LOD. At 12 days of treatment, the fecal TCC concentration increased to 2220 µg/g among 0.2% w/w exposed animals. The concentration in fecal samples decreased over the washout period in 0.2% w/w treated animals to 0.399 µ/g feces after exposure was removed for 28 days. This method required a small amount of sample (0.1 g) with simple sample preparation. Given its sensitivity and efficiency, this method may be useful for monitoring TCC exposure in toxicological studies of animals.

Original languageEnglish (US)
Pages (from-to)8125-8132
Number of pages8
JournalInternational journal of environmental research and public health
Volume12
Issue number7
DOIs
StatePublished - Jul 15 2015

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Mass Spectrometry
Feces
Pressure
Toxicology
Limit of Detection
High Pressure Liquid Chromatography
triclocarban
Urine
Water

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Extraction of 3,4,4'-trichlorocarbanilide from rat fecal samples for determination by high pressure liquid chromatography–tandem mass spectrometry. / Kennedy, Rebekah C.; Fling, Russell R.; Terry, Paul D.; Menn, Fu Min; Chen, Jiangang; Borman, Christopher J.

In: International journal of environmental research and public health, Vol. 12, No. 7, 15.07.2015, p. 8125-8132.

Research output: Contribution to journalArticle

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