Extraction of type II collagen and electrospinning nano-fibrous three-dimensional Scaffolds

Danielle C. Knapp, Catherine P. Barnes, David Brand, Matthew J. Bechnan, Gary L. Bowlin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Articular cartilage lining joints functions to reduce friction and absorb shock. Collagen type II is the largest constituent in the extracellular matrix of articular cartilage. Restoration of articular cartilage is of the highest interest to tissue engineers because this material has little ability to naturally regenerate due to the absence of vascularity and the inability of the chondrocytes to proliferate at a high rate. It would be ideal to create a mimicking extracellular matrix/scaffold from type II collagen that could possibly be used to replace the damaged articular cartilage that has both the function and morphology of natural articular cartilage. In this feasibility study, articular cartilage chips from three different vendors were utilized. The three groups are as follows: articular cartilage from fetal calf leg joints (Dr. David Brand), manually shaved clear hyaline cartilage chips from the surface of patellas from 140-180 day old calves (Spear Products), and pre-cut cartilage chunks that were more bluntly/deeply cut (leaving blood vessels and bone marrow fragments) from the patellas of 140-180 day old calves were utilized (Lampire Biological Laboratories, Inc.). The end product of collagen type II was then electrospun at concentrations of 0.06, 0.08,0.10, and 0.12 g/mL and both fiber diameter and pore size were analyzed.

Original languageEnglish (US)
Title of host publication"New Century, New Materials and New Life" - Proceedings of 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005
PublisherChina Press
Pages889-892
Number of pages4
ISBN (Print)7502577173, 9787502577179
StatePublished - Jan 1 2005
Event2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005 - Shanghai, China
Duration: Oct 19 2005Oct 21 2005

Publication series

Name"New Century, New Materials and New Life" - Proceedings of 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005
Volume2

Other

Other2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005
CountryChina
CityShanghai
Period10/19/0510/21/05

Fingerprint

Collagen Type II
Cartilage
Electrospinning
Scaffolds (biology)
Collagen
Scaffolds
Blood vessels
Linings
Restoration
Pore size
Bone
Tissue
Friction
Engineers
Fibers

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Polymers and Plastics

Cite this

Knapp, D. C., Barnes, C. P., Brand, D., Bechnan, M. J., & Bowlin, G. L. (2005). Extraction of type II collagen and electrospinning nano-fibrous three-dimensional Scaffolds. In "New Century, New Materials and New Life" - Proceedings of 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005 (pp. 889-892). ("New Century, New Materials and New Life" - Proceedings of 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005; Vol. 2). China Press.

Extraction of type II collagen and electrospinning nano-fibrous three-dimensional Scaffolds. / Knapp, Danielle C.; Barnes, Catherine P.; Brand, David; Bechnan, Matthew J.; Bowlin, Gary L.

"New Century, New Materials and New Life" - Proceedings of 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005. China Press, 2005. p. 889-892 ("New Century, New Materials and New Life" - Proceedings of 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Knapp, DC, Barnes, CP, Brand, D, Bechnan, MJ & Bowlin, GL 2005, Extraction of type II collagen and electrospinning nano-fibrous three-dimensional Scaffolds. in "New Century, New Materials and New Life" - Proceedings of 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005. "New Century, New Materials and New Life" - Proceedings of 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005, vol. 2, China Press, pp. 889-892, 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005, Shanghai, China, 10/19/05.
Knapp DC, Barnes CP, Brand D, Bechnan MJ, Bowlin GL. Extraction of type II collagen and electrospinning nano-fibrous three-dimensional Scaffolds. In "New Century, New Materials and New Life" - Proceedings of 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005. China Press. 2005. p. 889-892. ("New Century, New Materials and New Life" - Proceedings of 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005).
Knapp, Danielle C. ; Barnes, Catherine P. ; Brand, David ; Bechnan, Matthew J. ; Bowlin, Gary L. / Extraction of type II collagen and electrospinning nano-fibrous three-dimensional Scaffolds. "New Century, New Materials and New Life" - Proceedings of 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005. China Press, 2005. pp. 889-892 ("New Century, New Materials and New Life" - Proceedings of 2005 International Conference on Advanced Fibers and Polymer Materials, ICAFPM 2005).
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