Extractionless high-performance liquid chromatographic method for the simultaneous determination of piroxicam and 5′-hydroxypiroxicam in human plasma and urine

A. Avgerinos, S. Axarlis, Ioannis Dragatsis, Th Karidas, S. Malamataris

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A simple and rapid (extractionless) high-performance liquid chromatographic method with UV detection, at 330 nm, was developed for the simultaneous determination of piroxicam and its major metabolite, 5′-hydroxypiroxicam, in human plasma and urine. Acidified plasma and alkali-treated urine samples are used and naproxen is added as internal standard. The separation is performed at 40°C on a C18 Spherisorb column with acetonitrile-0.1 M sodium acetate (33:67, v/v, pH 3.3) as mobile phase. The retention time is 2.2 min for 5′-hydroxypiroxicam, 2.6 min for piroxicam and 3.2 min for naproxen. The detection limit is 0.05 μg/ml using a 100-μl loop.

Original languageEnglish (US)
Pages (from-to)142-146
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume673
Issue number1
DOIs
StatePublished - Nov 3 1995
Externally publishedYes

Fingerprint

Plasma (human)
Piroxicam
Naproxen
Sodium Acetate
Liquids
Alkalies
Metabolites
Plasmas
5'-hydroxypiroxicam
acetonitrile

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

@article{c66803f19c5949c08d240ecd91af441b,
title = "Extractionless high-performance liquid chromatographic method for the simultaneous determination of piroxicam and 5′-hydroxypiroxicam in human plasma and urine",
abstract = "A simple and rapid (extractionless) high-performance liquid chromatographic method with UV detection, at 330 nm, was developed for the simultaneous determination of piroxicam and its major metabolite, 5′-hydroxypiroxicam, in human plasma and urine. Acidified plasma and alkali-treated urine samples are used and naproxen is added as internal standard. The separation is performed at 40°C on a C18 Spherisorb column with acetonitrile-0.1 M sodium acetate (33:67, v/v, pH 3.3) as mobile phase. The retention time is 2.2 min for 5′-hydroxypiroxicam, 2.6 min for piroxicam and 3.2 min for naproxen. The detection limit is 0.05 μg/ml using a 100-μl loop.",
author = "A. Avgerinos and S. Axarlis and Ioannis Dragatsis and Th Karidas and S. Malamataris",
year = "1995",
month = "11",
day = "3",
doi = "10.1016/0378-4347(95)00248-H",
language = "English (US)",
volume = "673",
pages = "142--146",
journal = "Journal of Chromatography B: Biomedical Sciences and Applications",
issn = "0378-4347",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Extractionless high-performance liquid chromatographic method for the simultaneous determination of piroxicam and 5′-hydroxypiroxicam in human plasma and urine

AU - Avgerinos, A.

AU - Axarlis, S.

AU - Dragatsis, Ioannis

AU - Karidas, Th

AU - Malamataris, S.

PY - 1995/11/3

Y1 - 1995/11/3

N2 - A simple and rapid (extractionless) high-performance liquid chromatographic method with UV detection, at 330 nm, was developed for the simultaneous determination of piroxicam and its major metabolite, 5′-hydroxypiroxicam, in human plasma and urine. Acidified plasma and alkali-treated urine samples are used and naproxen is added as internal standard. The separation is performed at 40°C on a C18 Spherisorb column with acetonitrile-0.1 M sodium acetate (33:67, v/v, pH 3.3) as mobile phase. The retention time is 2.2 min for 5′-hydroxypiroxicam, 2.6 min for piroxicam and 3.2 min for naproxen. The detection limit is 0.05 μg/ml using a 100-μl loop.

AB - A simple and rapid (extractionless) high-performance liquid chromatographic method with UV detection, at 330 nm, was developed for the simultaneous determination of piroxicam and its major metabolite, 5′-hydroxypiroxicam, in human plasma and urine. Acidified plasma and alkali-treated urine samples are used and naproxen is added as internal standard. The separation is performed at 40°C on a C18 Spherisorb column with acetonitrile-0.1 M sodium acetate (33:67, v/v, pH 3.3) as mobile phase. The retention time is 2.2 min for 5′-hydroxypiroxicam, 2.6 min for piroxicam and 3.2 min for naproxen. The detection limit is 0.05 μg/ml using a 100-μl loop.

UR - http://www.scopus.com/inward/record.url?scp=0028866101&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028866101&partnerID=8YFLogxK

U2 - 10.1016/0378-4347(95)00248-H

DO - 10.1016/0378-4347(95)00248-H

M3 - Article

VL - 673

SP - 142

EP - 146

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

SN - 0378-4347

IS - 1

ER -