Fabrication of cardiac patch with decellularized porcine myocardial scaffold and bone marrow mononuclear cells

Bo Wang, Ali Borazjani, Mina Tahai, Amy Curry, Dan T. Simionescu, Jianjun Guan, Filip To, Steve H. Elder, Jun Liao

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Tissue engineered cardiac grafts are a promising therapeutic mode for ventricular wall reconstruction. Recently, it has been found that acellular tissue scaffolds provide natural ultrastructural, mechanical, and compositional cues for recellularization and tissue remodeling. We thus assess the potential of decellularized porcine myocardium as a scaffold for thick cardiac patch tissue engineering. Myocardial sections with 2-mm thickness were decellularized using 0.1% sodium dodecyl sulfate and then reseeded with differentiated bone marrow mononuclear cells. We found that thorough decellularization could be achieved after 2.5 weeks of treatment. Reseeded cells were found to infiltrate and proliferate in the tissue constructs. Immunohistological staining studies showed that the reseeded cells maintained cardiomyocyte-like phenotype and possible endothelialization was found in locations close to vasculature channels, indicating angiogenesis potential. Both biaxial and uniaxial mechanical testing showed a stiffer mechanical response of the acellular myocardial scaffolds; however, tissue extensibility and tensile modulus were found to recover in the constructs along with the culture time, as expected from increased cellular content. The cardiac patch that we envision for clinical application will benefit from the natural architecture of myocardial extracellular matrix, which has the potential to promote stem cell differentiation, cardiac regeneration, and angiogenesis.

Original languageEnglish (US)
Pages (from-to)1100-1110
Number of pages11
JournalJournal of Biomedical Materials Research - Part A
Volume94
Issue number4
DOIs
StatePublished - Sep 15 2010
Externally publishedYes

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Scaffolds
Bone
Tissue
Fabrication
Tissue Scaffolds
Mechanical testing
Sodium dodecyl sulfate
Scaffolds (biology)
Stem cells
Tissue engineering
Grafts
Sodium Dodecyl Sulfate
Elastic moduli

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Fabrication of cardiac patch with decellularized porcine myocardial scaffold and bone marrow mononuclear cells. / Wang, Bo; Borazjani, Ali; Tahai, Mina; Curry, Amy; Simionescu, Dan T.; Guan, Jianjun; To, Filip; Elder, Steve H.; Liao, Jun.

In: Journal of Biomedical Materials Research - Part A, Vol. 94, No. 4, 15.09.2010, p. 1100-1110.

Research output: Contribution to journalArticle

Wang, Bo ; Borazjani, Ali ; Tahai, Mina ; Curry, Amy ; Simionescu, Dan T. ; Guan, Jianjun ; To, Filip ; Elder, Steve H. ; Liao, Jun. / Fabrication of cardiac patch with decellularized porcine myocardial scaffold and bone marrow mononuclear cells. In: Journal of Biomedical Materials Research - Part A. 2010 ; Vol. 94, No. 4. pp. 1100-1110.
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