Factors regulating collagen synthesis and degradation during second-intention healing of wounds in the thoracic region and the distal aspect of the forelimb of horses

Anne J. Schwartz, David A. Wilson, Kevin G. Keegan, Venkataseshu K. Ganjam, Yao Sun, Karl Weber, Jiakun Zhang

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Objective - To determine significant molecular and cellular factors responsible for differences in second-intention healing in thoracic and metacarpal wounds of horses. Animals - 6 adult mixed-breed horses. Procedure - A full-thickness skin wound on the metacarpus and another such wound on the pectoral region were created, photographed, and measured and tissue was harvested from these sites weekly for 4 weeks. Gene expression of type-I collagen, transforming growth factor (TGF)-β1, matrix metalloproteinase (MMP)-1, and tissue inhibitor of metalloproteinase (TIMP)-1 were determined by quantitative in situ hybridization. Myofibroblasts were detected by immunolhistochemical labeling with α-smooth muscle actin (α-SMA). Collagen accumulation was detected by use of picrosirius red staining. Tissue morphology was examined by use of H&E staining. Results - Unlike thoracic wounds, forelimb wounds enlarged during the first 2 weeks. Myofibroblasts, detected by week 1, remained abundant with superior organization in thoracic wounds. Type-I collagen mRNA accumulated progressively in both wounds. More type-I collagen and TGF-β1 mRNA were seen in forelimb wounds. Volume of MMP-1 mRNA decreased from day 0 in both wounds. By week 3, TIMP-1 mRNA concentration was greater in thoracic wounds. Conclusions and Clinical Relevance - Greater collagen synthesis in metacarpal than thoracic wounds was documented by increased concentrations of myofibroblasts, type-I collagen mRNA,TGF-β1 mRNA, and decreased collagen degradation (ie, MMP-1). Imbalanced collagen synthesis and degradation likely correlate with development of exuberant granulation tissue, delaying healing in wounds of the distal portions of the limbs. Factors that inhibit collagen synthesis or stimulate collagenase may provide treatment options for horses with exuberant granulation tissue.

Original languageEnglish (US)
Pages (from-to)1564-1570
Number of pages7
JournalAmerican journal of veterinary research
Volume63
Issue number11
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

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Forelimb
chest
forelimbs
tissue repair
animal injuries
Wound Healing
Horses
collagen
Collagen
Thorax
horses
synthesis
degradation
Wounds and Injuries
interstitial collagenase
Collagen Type I
metacarpus
transforming growth factors
Messenger RNA
Matrix Metalloproteinase 1

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Factors regulating collagen synthesis and degradation during second-intention healing of wounds in the thoracic region and the distal aspect of the forelimb of horses. / Schwartz, Anne J.; Wilson, David A.; Keegan, Kevin G.; Ganjam, Venkataseshu K.; Sun, Yao; Weber, Karl; Zhang, Jiakun.

In: American journal of veterinary research, Vol. 63, No. 11, 01.11.2002, p. 1564-1570.

Research output: Contribution to journalArticle

Schwartz, Anne J. ; Wilson, David A. ; Keegan, Kevin G. ; Ganjam, Venkataseshu K. ; Sun, Yao ; Weber, Karl ; Zhang, Jiakun. / Factors regulating collagen synthesis and degradation during second-intention healing of wounds in the thoracic region and the distal aspect of the forelimb of horses. In: American journal of veterinary research. 2002 ; Vol. 63, No. 11. pp. 1564-1570.
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abstract = "Objective - To determine significant molecular and cellular factors responsible for differences in second-intention healing in thoracic and metacarpal wounds of horses. Animals - 6 adult mixed-breed horses. Procedure - A full-thickness skin wound on the metacarpus and another such wound on the pectoral region were created, photographed, and measured and tissue was harvested from these sites weekly for 4 weeks. Gene expression of type-I collagen, transforming growth factor (TGF)-β1, matrix metalloproteinase (MMP)-1, and tissue inhibitor of metalloproteinase (TIMP)-1 were determined by quantitative in situ hybridization. Myofibroblasts were detected by immunolhistochemical labeling with α-smooth muscle actin (α-SMA). Collagen accumulation was detected by use of picrosirius red staining. Tissue morphology was examined by use of H&E staining. Results - Unlike thoracic wounds, forelimb wounds enlarged during the first 2 weeks. Myofibroblasts, detected by week 1, remained abundant with superior organization in thoracic wounds. Type-I collagen mRNA accumulated progressively in both wounds. More type-I collagen and TGF-β1 mRNA were seen in forelimb wounds. Volume of MMP-1 mRNA decreased from day 0 in both wounds. By week 3, TIMP-1 mRNA concentration was greater in thoracic wounds. Conclusions and Clinical Relevance - Greater collagen synthesis in metacarpal than thoracic wounds was documented by increased concentrations of myofibroblasts, type-I collagen mRNA,TGF-β1 mRNA, and decreased collagen degradation (ie, MMP-1). Imbalanced collagen synthesis and degradation likely correlate with development of exuberant granulation tissue, delaying healing in wounds of the distal portions of the limbs. Factors that inhibit collagen synthesis or stimulate collagenase may provide treatment options for horses with exuberant granulation tissue.",
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AU - Keegan, Kevin G.

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AU - Sun, Yao

AU - Weber, Karl

AU - Zhang, Jiakun

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AB - Objective - To determine significant molecular and cellular factors responsible for differences in second-intention healing in thoracic and metacarpal wounds of horses. Animals - 6 adult mixed-breed horses. Procedure - A full-thickness skin wound on the metacarpus and another such wound on the pectoral region were created, photographed, and measured and tissue was harvested from these sites weekly for 4 weeks. Gene expression of type-I collagen, transforming growth factor (TGF)-β1, matrix metalloproteinase (MMP)-1, and tissue inhibitor of metalloproteinase (TIMP)-1 were determined by quantitative in situ hybridization. Myofibroblasts were detected by immunolhistochemical labeling with α-smooth muscle actin (α-SMA). Collagen accumulation was detected by use of picrosirius red staining. Tissue morphology was examined by use of H&E staining. Results - Unlike thoracic wounds, forelimb wounds enlarged during the first 2 weeks. Myofibroblasts, detected by week 1, remained abundant with superior organization in thoracic wounds. Type-I collagen mRNA accumulated progressively in both wounds. More type-I collagen and TGF-β1 mRNA were seen in forelimb wounds. Volume of MMP-1 mRNA decreased from day 0 in both wounds. By week 3, TIMP-1 mRNA concentration was greater in thoracic wounds. Conclusions and Clinical Relevance - Greater collagen synthesis in metacarpal than thoracic wounds was documented by increased concentrations of myofibroblasts, type-I collagen mRNA,TGF-β1 mRNA, and decreased collagen degradation (ie, MMP-1). Imbalanced collagen synthesis and degradation likely correlate with development of exuberant granulation tissue, delaying healing in wounds of the distal portions of the limbs. Factors that inhibit collagen synthesis or stimulate collagenase may provide treatment options for horses with exuberant granulation tissue.

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