False-positive reactions with enzyme-linked immunosorbent assay of Mycobacterium tuberculosis antigens in pleural fluid

Rajiv Dhand, N. K. Ganguly, C. Vaishnavi, R. Gilhotra, S. K. Malik

Research output: Contribution to journalArticle

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Abstract

The value of enzyme-linked immunosorbent assay (ELISA) for the diagnosis of tuberculous pleural effusion has not been defined. We performed ELISA by a double antibody sandwich technique with anti-BCG antibody in the solid phase to detect Mycobacterium tuberculosis antigen in pleural fluid from 36 patients with pleural effusion (tuberculosis 15, lung cancer 12, miscellaneous 9). Pleural fluids from 12 of the tuberculosis patients, 12 of the cancer patients and one patient in the miscellaneous group had optical densities above the cut-off point. False-positive reactions in patients with lung cancer limit the usefulness of ELISA with conventional anti-BCG antibody for detection of M. tuberculosis antigen.

Original languageEnglish (US)
Pages (from-to)241-243
Number of pages3
JournalJournal of Medical Microbiology
Volume26
Issue number4
DOIs
StatePublished - Jan 1 1988

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False Positive Reactions
Enzyme-Linked Immunosorbent Assay
Pleural Effusion
Mycobacterium bovis
Anti-Idiotypic Antibodies
Lung Neoplasms
Pleural Tuberculosis
Tuberculosis
Mycobacterium tuberculosis antigens
Antibodies
Neoplasms

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

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False-positive reactions with enzyme-linked immunosorbent assay of Mycobacterium tuberculosis antigens in pleural fluid. / Dhand, Rajiv; Ganguly, N. K.; Vaishnavi, C.; Gilhotra, R.; Malik, S. K.

In: Journal of Medical Microbiology, Vol. 26, No. 4, 01.01.1988, p. 241-243.

Research output: Contribution to journalArticle

Dhand, Rajiv ; Ganguly, N. K. ; Vaishnavi, C. ; Gilhotra, R. ; Malik, S. K. / False-positive reactions with enzyme-linked immunosorbent assay of Mycobacterium tuberculosis antigens in pleural fluid. In: Journal of Medical Microbiology. 1988 ; Vol. 26, No. 4. pp. 241-243.
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