Fast atom bombardment mass spectrometry of synthetic peptides

Sarka Beranova, Dominic M. Desiderio

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Fast atom bombardment mass spectrometry plays a continuing and effective role in the rapid and efficient analysis of synthetic peptides. In this article, the basic principles of FAB-MS and FAB-MS/MS are reviewed, and the limitations and pitfalls of the method are discussed. The potential of the technique is illustrated by several selected applications. The molecular weight of a synthetic peptide can be readily and accurately determined by FAB-MS. The sensitivity of the FAB-MS method also makes it extremely useful for the evaluation of the purity of a peptide. FAB-MS/MS allows the elucidation of the primary structure of the target peptide, even in a mixture, and also permits the rapid identification of synthetic side products. Hence, FAB-MS and FAB-MS/MS aid in the unequivocal characterization of synthetic peptides. Furthermore, the information that is gained from the FAB analysis can help to unravel potential problems that may be associated with the synthesis.

Original languageEnglish (US)
Pages (from-to)478-499
Number of pages22
JournalMethods in enzymology
Volume289
DOIs
StatePublished - Jan 1 1997

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Fast Atom Bombardment Mass Spectrometry
Mass spectrometry
Atoms
Peptides
Molecular Weight
Molecular weight

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Fast atom bombardment mass spectrometry of synthetic peptides. / Beranova, Sarka; Desiderio, Dominic M.

In: Methods in enzymology, Vol. 289, 01.01.1997, p. 478-499.

Research output: Contribution to journalArticle

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