Featured Article

Effect of copper on nuclear translocation of copper chaperone for superoxide dismutase-1

Lin Wang, Yan Ge, Yujian Kang

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5 Citations (Scopus)

Abstract

Copper chaperone for superoxide dismutase-1 (CCS-1), facilitating copper insertion into superoxide dismutase 1 (SOD-1), is present in the nucleus. However, it is unknown how CCS-1 is translocated to the nucleus. The present study was undertaken to determine the effect of copper on nuclear translocation of CCS-1. Human umbilical vein endothelial cells (HUVECs) were subjected to hypoxia, causing an increase in both copper and CCS-1 in the nucleus. Treatment with tetraethylenepentamine (TEPA) not only decreased the total cellular concentration and the nuclear translocation of copper, but also completely suppressed the entry of CCS-1 to the nucleus. On the other hand, siRNA targeting CCS-1 neither inhibited the increase in total concentrations nor blocked the nuclear translocation of copper. This study thus demonstrates that under hypoxia condition, both copper and CCS-1 are transported to the nucleus. The nuclear translocation of CCS-1 is copper dependent, but the nuclear translocation of copper could take place alternatively in a CCS-1-independent pathway.

Original languageEnglish (US)
Pages (from-to)1483-1488
Number of pages6
JournalExperimental Biology and Medicine
Volume241
Issue number14
DOIs
StatePublished - Aug 1 2016
Externally publishedYes

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Superoxide Dismutase
Copper
Superoxide Dismutase-1
Endothelial cells
Human Umbilical Vein Endothelial Cells
Small Interfering RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Featured Article : Effect of copper on nuclear translocation of copper chaperone for superoxide dismutase-1. / Wang, Lin; Ge, Yan; Kang, Yujian.

In: Experimental Biology and Medicine, Vol. 241, No. 14, 01.08.2016, p. 1483-1488.

Research output: Contribution to journalArticle

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