Fetal cells in the maternal circulation

Isolation by multiparameter flow cytometry and confirmation by polymerase chain reaction

Stephen Wachtel, Sherman Elias, James Price, Gwendolyn Wachtel, Owen Phillips, Lee Shulman, Carole Meyers, Joe Leigh Simpson, Michael Dockter

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

During pregnancy, nucleated fetal erythrocytes enter the maternal circulation and can be isolated efficiently from the maternal cells by multiparameter flow cytometry. Male DNA, implying presence of a male fetus, can be identified in flowsorted maternal blood by polymerase chain reaction with oligonucleotide primers flanking single-copy Y-specific DNA sequences. Among flow-sorted samples, we correctly identified fetal sex in 17/18 (94%) pregnancies of 10-21 weeks gestation. Maternal blood thus provides a potential opportunity for prenatal diagnosis that could preclude the need for invasive procedures in current use.

Original languageEnglish (US)
Pages (from-to)1466-1469
Number of pages4
JournalHuman Reproduction
Volume6
Issue number10
DOIs
StatePublished - Jan 1 1991

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Flow Cytometry
Mothers
Polymerase Chain Reaction
Pregnancy
Erythroblasts
DNA Primers
Prenatal Diagnosis
Fetus
DNA

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Fetal cells in the maternal circulation : Isolation by multiparameter flow cytometry and confirmation by polymerase chain reaction. / Wachtel, Stephen; Elias, Sherman; Price, James; Wachtel, Gwendolyn; Phillips, Owen; Shulman, Lee; Meyers, Carole; Simpson, Joe Leigh; Dockter, Michael.

In: Human Reproduction, Vol. 6, No. 10, 01.01.1991, p. 1466-1469.

Research output: Contribution to journalArticle

Wachtel, Stephen ; Elias, Sherman ; Price, James ; Wachtel, Gwendolyn ; Phillips, Owen ; Shulman, Lee ; Meyers, Carole ; Simpson, Joe Leigh ; Dockter, Michael. / Fetal cells in the maternal circulation : Isolation by multiparameter flow cytometry and confirmation by polymerase chain reaction. In: Human Reproduction. 1991 ; Vol. 6, No. 10. pp. 1466-1469.
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