Fibroblast stimulation in schistosomiasis. VII. Egg granulomas secrete factors that stimulate collagen and fibronectin synthesis

D. J. Wyler, H. P. Ehrlich, Arnold Postlethwaite, Rajendra Raghow, M. M. Murphy

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Abstract

Tissue fibrosis in schistosomiasis is largely responsible for the important morbidity that results from infection with the trematode worms, Schistosoma. Neither the migrating larval forms (cercariae) nor the intravascular adult worms appear to incite pathological responses that are important in chronic schistosomiasis. On the other hand, eggs deposited in tissue incite chronic granulomatous inflammatory responses that are the hallmark of infection and precede the onset of adjacent tissue fibrosis. We previously reported that products of the egg granulomas can stimulate a number of relevant responses in fibroblast cultures that in vivo would be expected to promote tissue fibrosis. We report here that the granulomas secrete factors that in vitro can stimulate collagen and fibronectin synthesis in fibroblasts. We determined that activity stimulating collagen synthesis is ≃10 Kd (gel filtration) with a pI of ≃5.5 (isoelectric focusing); additional activity is also detected in some other fractions (≃33 Kd; pI ~7.0). In contrast, the activity stimulating fibronectin synthesis was ≃22 Kd with a pI of 5.5. Activity was also present in fractions of 50 Kd with pI of ~7.5. Fibroblasts grown in granuloma supernatant-containing medium contained greater steady-state levels of specific mRNA coding for type I procollagen and fibronectin compared with cells cultured in unsupplemented medium. These observations support the hypothesis that biologically active molecules secreted by granuloma cells are instrumental in the initiation of tissue fibrosis in schistosomiasis.

Original languageEnglish (US)
Pages (from-to)1581-1586
Number of pages6
JournalJournal of Immunology
Volume138
Issue number5
StatePublished - May 5 1987

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Schistosomiasis
Granuloma
Fibronectins
Ovum
Collagen
Fibroblasts
Fibrosis
Trematode Infections
Schistosoma
Cercaria
Isoelectric Focusing
Collagen Type I
Eggs
Gel Chromatography
Cultured Cells
Morbidity
Messenger RNA
Infection

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Fibroblast stimulation in schistosomiasis. VII. Egg granulomas secrete factors that stimulate collagen and fibronectin synthesis. / Wyler, D. J.; Ehrlich, H. P.; Postlethwaite, Arnold; Raghow, Rajendra; Murphy, M. M.

In: Journal of Immunology, Vol. 138, No. 5, 05.05.1987, p. 1581-1586.

Research output: Contribution to journalArticle

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