Flow cytometer analysis of oral premalignant lesions

a pilot study and review

Michael A. Kahn, Michael E. Dockter, Jeanne M. Hermann‐Petrin

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    In a retrospective study, we examined 34 premalignant lesions of the oral cavity by flow cytometer analysis on formalin‐fixed, paraffin‐embedded tissue submitted to the University of Tennessee. Memphis, Oral Pathology Laboratory. Three categories of oral epithelial dysplasia were represented (eight mild, seven moderate, nine severe), as well as live epithelial hyperplasias without dysplasia and five fibrous nodules as controls. The DNA index and total proliferative index of each ease were calculated. The objective of the study was to determine the amount of epithelial dysplasia necessary in oral lesions before DNA aneuploidy or high proliferative index is detectable and thus determine if How cytometric analysis can be a diagnostic adjunct for oral premalignant lesions. The results showed that some cases in both the control and dysplastic categories exhibited a high total proliferative index (control = 1, no dysplasia = 1, mild dysplasia = 3, moderate dysplasia = 2, severe dysplasia = 2), whereas only the dysplastic lesions had an abnormal DNA index [8 of 24 (33%)]. The results indicate that flow cytometric analysis may have some limited potential as a diagnostic adjunct in oral premalignant lesions.

    Original languageEnglish (US)
    Pages (from-to)1-6
    Number of pages6
    JournalJournal of Oral Pathology & Medicine
    Volume21
    Issue number1
    DOIs
    StatePublished - 1992

    Fingerprint

    DNA
    Oral Pathology
    Aneuploidy
    Hyperplasia
    Mouth
    Retrospective Studies

    All Science Journal Classification (ASJC) codes

    • Pathology and Forensic Medicine
    • Oral Surgery
    • Otorhinolaryngology
    • Cancer Research
    • Periodontics

    Cite this

    Flow cytometer analysis of oral premalignant lesions : a pilot study and review. / Kahn, Michael A.; Dockter, Michael E.; Hermann‐Petrin, Jeanne M.

    In: Journal of Oral Pathology & Medicine, Vol. 21, No. 1, 1992, p. 1-6.

    Research output: Contribution to journalArticle

    Kahn, Michael A. ; Dockter, Michael E. ; Hermann‐Petrin, Jeanne M. / Flow cytometer analysis of oral premalignant lesions : a pilot study and review. In: Journal of Oral Pathology & Medicine. 1992 ; Vol. 21, No. 1. pp. 1-6.
    @article{3fd4e76a8f4446d29ca12820b97429ae,
    title = "Flow cytometer analysis of oral premalignant lesions: a pilot study and review",
    abstract = "In a retrospective study, we examined 34 premalignant lesions of the oral cavity by flow cytometer analysis on formalin‐fixed, paraffin‐embedded tissue submitted to the University of Tennessee. Memphis, Oral Pathology Laboratory. Three categories of oral epithelial dysplasia were represented (eight mild, seven moderate, nine severe), as well as live epithelial hyperplasias without dysplasia and five fibrous nodules as controls. The DNA index and total proliferative index of each ease were calculated. The objective of the study was to determine the amount of epithelial dysplasia necessary in oral lesions before DNA aneuploidy or high proliferative index is detectable and thus determine if How cytometric analysis can be a diagnostic adjunct for oral premalignant lesions. The results showed that some cases in both the control and dysplastic categories exhibited a high total proliferative index (control = 1, no dysplasia = 1, mild dysplasia = 3, moderate dysplasia = 2, severe dysplasia = 2), whereas only the dysplastic lesions had an abnormal DNA index [8 of 24 (33{\%})]. The results indicate that flow cytometric analysis may have some limited potential as a diagnostic adjunct in oral premalignant lesions.",
    author = "Kahn, {Michael A.} and Dockter, {Michael E.} and Hermann‐Petrin, {Jeanne M.}",
    year = "1992",
    doi = "10.1111/j.1600-0714.1992.tb00959.x",
    language = "English (US)",
    volume = "21",
    pages = "1--6",
    journal = "Journal of Oral Pathology and Medicine",
    issn = "0904-2512",
    publisher = "Wiley-Blackwell",
    number = "1",

    }

    TY - JOUR

    T1 - Flow cytometer analysis of oral premalignant lesions

    T2 - a pilot study and review

    AU - Kahn, Michael A.

    AU - Dockter, Michael E.

    AU - Hermann‐Petrin, Jeanne M.

    PY - 1992

    Y1 - 1992

    N2 - In a retrospective study, we examined 34 premalignant lesions of the oral cavity by flow cytometer analysis on formalin‐fixed, paraffin‐embedded tissue submitted to the University of Tennessee. Memphis, Oral Pathology Laboratory. Three categories of oral epithelial dysplasia were represented (eight mild, seven moderate, nine severe), as well as live epithelial hyperplasias without dysplasia and five fibrous nodules as controls. The DNA index and total proliferative index of each ease were calculated. The objective of the study was to determine the amount of epithelial dysplasia necessary in oral lesions before DNA aneuploidy or high proliferative index is detectable and thus determine if How cytometric analysis can be a diagnostic adjunct for oral premalignant lesions. The results showed that some cases in both the control and dysplastic categories exhibited a high total proliferative index (control = 1, no dysplasia = 1, mild dysplasia = 3, moderate dysplasia = 2, severe dysplasia = 2), whereas only the dysplastic lesions had an abnormal DNA index [8 of 24 (33%)]. The results indicate that flow cytometric analysis may have some limited potential as a diagnostic adjunct in oral premalignant lesions.

    AB - In a retrospective study, we examined 34 premalignant lesions of the oral cavity by flow cytometer analysis on formalin‐fixed, paraffin‐embedded tissue submitted to the University of Tennessee. Memphis, Oral Pathology Laboratory. Three categories of oral epithelial dysplasia were represented (eight mild, seven moderate, nine severe), as well as live epithelial hyperplasias without dysplasia and five fibrous nodules as controls. The DNA index and total proliferative index of each ease were calculated. The objective of the study was to determine the amount of epithelial dysplasia necessary in oral lesions before DNA aneuploidy or high proliferative index is detectable and thus determine if How cytometric analysis can be a diagnostic adjunct for oral premalignant lesions. The results showed that some cases in both the control and dysplastic categories exhibited a high total proliferative index (control = 1, no dysplasia = 1, mild dysplasia = 3, moderate dysplasia = 2, severe dysplasia = 2), whereas only the dysplastic lesions had an abnormal DNA index [8 of 24 (33%)]. The results indicate that flow cytometric analysis may have some limited potential as a diagnostic adjunct in oral premalignant lesions.

    UR - http://www.scopus.com/inward/record.url?scp=0026516765&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0026516765&partnerID=8YFLogxK

    U2 - 10.1111/j.1600-0714.1992.tb00959.x

    DO - 10.1111/j.1600-0714.1992.tb00959.x

    M3 - Article

    VL - 21

    SP - 1

    EP - 6

    JO - Journal of Oral Pathology and Medicine

    JF - Journal of Oral Pathology and Medicine

    SN - 0904-2512

    IS - 1

    ER -