Fluorescence imaging of mycobacterial infection in live mice using fluorescent protein-expressing strains

Ying Kong, Jeffrey D. Cirillo

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Fluorescence imaging has been applied to various areas of biological research, including studies of physiological, neurological, oncological, cell biological, molecular, developmental, immunological, and infectious processes. In this chapter, we describe methods of fluorescent imaging applied to examination of subcutaneous and pulmonary mycobacterial infections in an animal model. Since slow growth of Mycobacterium tuberculosis (Mtb) hinders development of new diagnostics, therapeutics, and vaccines for tuberculosis (TB), we developed fluorescent protein (FP) expressing mycobacterial strains for in vivo imaging, which can be used to track bacterial location and to quantitate bacterial load directly in living animals. After comparison of imaging data using strains expressing different fluorescent proteins, we found that strains expressing L5-tdTomato display the greatest fluorescence. Here, we describe detailed protocols for tdTomato-labeled M. bovis BCG imaging in real time for subcutaneous and pulmonary infections in living mice. These procedures allow rapid and accurate determination of bacterial numbers in live mice.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages75-85
Number of pages11
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1790
ISSN (Print)1064-3745

Fingerprint

Optical Imaging
Tuberculosis Vaccines
Lung
Bacterial Load
Mycobacterium bovis
Infection
Mycobacterium tuberculosis
Proteins
Animal Models
Fluorescence
Growth
Research
Therapeutics

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Kong, Y., & Cirillo, J. D. (2018). Fluorescence imaging of mycobacterial infection in live mice using fluorescent protein-expressing strains. In Methods in Molecular Biology (pp. 75-85). (Methods in Molecular Biology; Vol. 1790). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7860-1_6

Fluorescence imaging of mycobacterial infection in live mice using fluorescent protein-expressing strains. / Kong, Ying; Cirillo, Jeffrey D.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 75-85 (Methods in Molecular Biology; Vol. 1790).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kong, Y & Cirillo, JD 2018, Fluorescence imaging of mycobacterial infection in live mice using fluorescent protein-expressing strains. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1790, Humana Press Inc., pp. 75-85. https://doi.org/10.1007/978-1-4939-7860-1_6
Kong Y, Cirillo JD. Fluorescence imaging of mycobacterial infection in live mice using fluorescent protein-expressing strains. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 75-85. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7860-1_6
Kong, Ying ; Cirillo, Jeffrey D. / Fluorescence imaging of mycobacterial infection in live mice using fluorescent protein-expressing strains. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 75-85 (Methods in Molecular Biology).
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