Fluoride inhibits the response of bone cells to mechanical loading

Hubertine Willems, Ellen G.H.M. van den Heuvel, Seb Castelein, Joost Keverling Buisman, Antonius L.J.J. Bronckers, Astrid D. Bakker, Jenneke Klein-Nulend

Research output: Contribution to journalArticle

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Abstract

The response of bone cells to mechanical loading is mediated by the cytoskeleton. Since the bone anabolic agent fluoride disrupts the cytoskeleton, we investigated whether fluoride affects the response of bone cells to mechanical loading, and whether this is cytoskeleton mediated. The mechano-response of osteoblasts was assessed in vitro by measuring pulsating fluid flow-induced nitric oxide (NO) production. Osteocyte shape was determined in hamster mandibles in vivo as parameter of osteocyte mechanosensitivity. Pulsating fluid flow (0.7 ± 0.3 Pa, 5 Hz) stimulated NO production by 8-fold within 5 min. NaF (10-50 μM) inhibited pulsating fluid flow-stimulated NO production after 10 min, and decreased F-actin content by ~3-fold. Fluid flow-induced NO response was also inhibited after F-actin disruption by cytochalasin B. NaF treatment resulted in more elongated, smaller osteocytes in interdental bone in vivo. Our results suggest that fluoride inhibits the mechano-response of bone cells, which might occur via cytoskeletal changes. Since decreased mechanosensitivity reduces bone mass, the reported anabolic effect of fluoride on bone mass in vivo is likely mediated by other factors than changed bone cell mechanosensitivity.

Original languageEnglish (US)
Pages (from-to)112-118
Number of pages7
JournalOdontology
Volume99
Issue number2
DOIs
StatePublished - Jul 1 2011

Fingerprint

Fluorides
Bone and Bones
Osteocytes
Pulsatile Flow
Nitric Oxide
Cytoskeleton
Anabolic Agents
Actins
Cytochalasin B
Osteoblasts
Mandible
Cricetinae

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Willems, H., van den Heuvel, E. G. H. M., Castelein, S., Buisman, J. K., Bronckers, A. L. J. J., Bakker, A. D., & Klein-Nulend, J. (2011). Fluoride inhibits the response of bone cells to mechanical loading. Odontology, 99(2), 112-118. https://doi.org/10.1007/s10266-011-0013-6

Fluoride inhibits the response of bone cells to mechanical loading. / Willems, Hubertine; van den Heuvel, Ellen G.H.M.; Castelein, Seb; Buisman, Joost Keverling; Bronckers, Antonius L.J.J.; Bakker, Astrid D.; Klein-Nulend, Jenneke.

In: Odontology, Vol. 99, No. 2, 01.07.2011, p. 112-118.

Research output: Contribution to journalArticle

Willems, H, van den Heuvel, EGHM, Castelein, S, Buisman, JK, Bronckers, ALJJ, Bakker, AD & Klein-Nulend, J 2011, 'Fluoride inhibits the response of bone cells to mechanical loading', Odontology, vol. 99, no. 2, pp. 112-118. https://doi.org/10.1007/s10266-011-0013-6
Willems H, van den Heuvel EGHM, Castelein S, Buisman JK, Bronckers ALJJ, Bakker AD et al. Fluoride inhibits the response of bone cells to mechanical loading. Odontology. 2011 Jul 1;99(2):112-118. https://doi.org/10.1007/s10266-011-0013-6
Willems, Hubertine ; van den Heuvel, Ellen G.H.M. ; Castelein, Seb ; Buisman, Joost Keverling ; Bronckers, Antonius L.J.J. ; Bakker, Astrid D. ; Klein-Nulend, Jenneke. / Fluoride inhibits the response of bone cells to mechanical loading. In: Odontology. 2011 ; Vol. 99, No. 2. pp. 112-118.
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