Formation of Odontoblast-Like Cells from Cultured Human Dental Pulp Cells on Dentin In Vitro

George T.J. Huang, Kristina Shagramanova, Selina W. Chan

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Recent characterization of human dental pulp stem cells has shed new light on the understanding of the odontoblastic lineage. The purpose of the study was to characterize human adult dental pulp cells isolated and cultured in vitro and to examine the cell differentiation potential grown on dentin. We observed that some pulp cells isolated with an enzyme-digestion approach proliferated at a similar rate as the immortal cell line NIH 3T3. Population doubling time (PDt) for pulp cells at passage 3 was 22.6 ± 0.5 hours and for NIH 3T3 was 23.1 ± 2.3 hours. The pulp cells formed mineral nodules stimulated with dexamethasone or dexamethasone plus 1,25-dihydroxyvitamin D3. Pulp cells, after being seeded onto mechanically and chemically treated dentin surface, appeared to establish an odontoblast-like morphology with a cytoplasmic process extending into a dentinal tubule revealed by scanning electron microscopy analysis. Our data demonstrated the formation of cells with odontoblastic morphologies on existing dentin, suggesting that isolated human pulp stem cells may differentiate into odontoblasts on dentin in vitro.

Original languageEnglish (US)
Pages (from-to)1066-1073
Number of pages8
JournalJournal of endodontics
Volume32
Issue number11
DOIs
StatePublished - Nov 1 2006

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Odontoblasts
Dental Pulp
Dentin
Cultured Cells
Dexamethasone
Stem Cells
Calcitriol
Electron Scanning Microscopy
Minerals
Cell Differentiation
Digestion
In Vitro Techniques
Cell Line
Enzymes
Population

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Formation of Odontoblast-Like Cells from Cultured Human Dental Pulp Cells on Dentin In Vitro. / Huang, George T.J.; Shagramanova, Kristina; Chan, Selina W.

In: Journal of endodontics, Vol. 32, No. 11, 01.11.2006, p. 1066-1073.

Research output: Contribution to journalArticle

Huang, George T.J. ; Shagramanova, Kristina ; Chan, Selina W. / Formation of Odontoblast-Like Cells from Cultured Human Dental Pulp Cells on Dentin In Vitro. In: Journal of endodontics. 2006 ; Vol. 32, No. 11. pp. 1066-1073.
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