Forskolin infusion in vivo increases ouabain binding in brain

Alan C. Swann, Jeffery Steketee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In order to investigate the possible regulation of brain Na,K-ATPase by cyclic AMP, we measured Na,K-ATPase activity and ouabain binding in cerebral cortex after intraventricular infusion of forskolin for 7 days. There was a dose-related increase in high-affinity ouabain binding, with a 75% increase at 12.5 μg/h forskolin. The effect on total enzyme activity was smaller but enzyme activity with high affinity for ouabain was increased by 65%, suggesting a selective effect on enzyme with high affinity for ouabain. Forskolin appeared not to interact directly with Na,K-ATPase in vitro.

Original languageEnglish (US)
Pages (from-to)351-353
Number of pages3
JournalBrain Research
Volume476
Issue number2
DOIs
StatePublished - Jan 9 1989

Fingerprint

Colforsin
Ouabain
Brain
Enzymes
Intraventricular Infusions
Cerebral Cortex
Cyclic AMP
sodium-translocating ATPase

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Forskolin infusion in vivo increases ouabain binding in brain. / Swann, Alan C.; Steketee, Jeffery.

In: Brain Research, Vol. 476, No. 2, 09.01.1989, p. 351-353.

Research output: Contribution to journalArticle

@article{2b399e2c786549c2aeed513275243c3d,
title = "Forskolin infusion in vivo increases ouabain binding in brain",
abstract = "In order to investigate the possible regulation of brain Na,K-ATPase by cyclic AMP, we measured Na,K-ATPase activity and ouabain binding in cerebral cortex after intraventricular infusion of forskolin for 7 days. There was a dose-related increase in high-affinity ouabain binding, with a 75{\%} increase at 12.5 μg/h forskolin. The effect on total enzyme activity was smaller but enzyme activity with high affinity for ouabain was increased by 65{\%}, suggesting a selective effect on enzyme with high affinity for ouabain. Forskolin appeared not to interact directly with Na,K-ATPase in vitro.",
author = "Swann, {Alan C.} and Jeffery Steketee",
year = "1989",
month = "1",
day = "9",
doi = "10.1016/0006-8993(89)91257-2",
language = "English (US)",
volume = "476",
pages = "351--353",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Forskolin infusion in vivo increases ouabain binding in brain

AU - Swann, Alan C.

AU - Steketee, Jeffery

PY - 1989/1/9

Y1 - 1989/1/9

N2 - In order to investigate the possible regulation of brain Na,K-ATPase by cyclic AMP, we measured Na,K-ATPase activity and ouabain binding in cerebral cortex after intraventricular infusion of forskolin for 7 days. There was a dose-related increase in high-affinity ouabain binding, with a 75% increase at 12.5 μg/h forskolin. The effect on total enzyme activity was smaller but enzyme activity with high affinity for ouabain was increased by 65%, suggesting a selective effect on enzyme with high affinity for ouabain. Forskolin appeared not to interact directly with Na,K-ATPase in vitro.

AB - In order to investigate the possible regulation of brain Na,K-ATPase by cyclic AMP, we measured Na,K-ATPase activity and ouabain binding in cerebral cortex after intraventricular infusion of forskolin for 7 days. There was a dose-related increase in high-affinity ouabain binding, with a 75% increase at 12.5 μg/h forskolin. The effect on total enzyme activity was smaller but enzyme activity with high affinity for ouabain was increased by 65%, suggesting a selective effect on enzyme with high affinity for ouabain. Forskolin appeared not to interact directly with Na,K-ATPase in vitro.

UR - http://www.scopus.com/inward/record.url?scp=0024562358&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024562358&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(89)91257-2

DO - 10.1016/0006-8993(89)91257-2

M3 - Article

VL - 476

SP - 351

EP - 353

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -