Free epsilon amino groups and 5-hydroxymethylfurfural contents in clear and cataractous human lenses

Rao Gadiparthi, E. Cotlier

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The free ε-amino groups and 5-hydroxymethylfurfural (5-HMF) contents were determined in soluble and insoluble proteins of clear human lenses and diabetic and nondiabetic senile cataractous lenses. The free ε-amino group content of soluble proteins in diabetic cataracts was decreased by 37% (P<0.01), whereas in nondiabetic senile cataracts it did not differ from that of clear lenses. The free ε-amino group content of insoluble proteins both in diabetic and nondiabetic cataracts was decreased significantly (P<0.001, P<0.015, respectively). The 5-HMF content of soluble proteins in diabetic cataracts was increased by 52% (P<0.001), whereas in nondiabetic cataracts it did not change from that of clear lenses. The 5-HMF content of insoluble proteins in diabetic as well as in nondiabetic cataracts was increased significantly as compared to that of clear lenses (P<0.001, P<0.001, respectively). The soluble protein of diabetic and nondiabetic cataracts was decreased with an increase in the insoluble protein content. These results suggest that nonenzymatic glycosylation plays a role in the conformational change of lens proteins in both diabetic and nondiabetic cataracts.

Original languageEnglish (US)
Pages (from-to)98-102
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume27
Issue number1
StatePublished - 1986
Externally publishedYes

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Cataract
Lenses
Proteins
Crystallins
5-hydroxymethylfurfural
Glycosylation

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

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abstract = "The free ε-amino groups and 5-hydroxymethylfurfural (5-HMF) contents were determined in soluble and insoluble proteins of clear human lenses and diabetic and nondiabetic senile cataractous lenses. The free ε-amino group content of soluble proteins in diabetic cataracts was decreased by 37{\%} (P<0.01), whereas in nondiabetic senile cataracts it did not differ from that of clear lenses. The free ε-amino group content of insoluble proteins both in diabetic and nondiabetic cataracts was decreased significantly (P<0.001, P<0.015, respectively). The 5-HMF content of soluble proteins in diabetic cataracts was increased by 52{\%} (P<0.001), whereas in nondiabetic cataracts it did not change from that of clear lenses. The 5-HMF content of insoluble proteins in diabetic as well as in nondiabetic cataracts was increased significantly as compared to that of clear lenses (P<0.001, P<0.001, respectively). The soluble protein of diabetic and nondiabetic cataracts was decreased with an increase in the insoluble protein content. These results suggest that nonenzymatic glycosylation plays a role in the conformational change of lens proteins in both diabetic and nondiabetic cataracts.",
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AU - Gadiparthi, Rao

AU - Cotlier, E.

PY - 1986

Y1 - 1986

N2 - The free ε-amino groups and 5-hydroxymethylfurfural (5-HMF) contents were determined in soluble and insoluble proteins of clear human lenses and diabetic and nondiabetic senile cataractous lenses. The free ε-amino group content of soluble proteins in diabetic cataracts was decreased by 37% (P<0.01), whereas in nondiabetic senile cataracts it did not differ from that of clear lenses. The free ε-amino group content of insoluble proteins both in diabetic and nondiabetic cataracts was decreased significantly (P<0.001, P<0.015, respectively). The 5-HMF content of soluble proteins in diabetic cataracts was increased by 52% (P<0.001), whereas in nondiabetic cataracts it did not change from that of clear lenses. The 5-HMF content of insoluble proteins in diabetic as well as in nondiabetic cataracts was increased significantly as compared to that of clear lenses (P<0.001, P<0.001, respectively). The soluble protein of diabetic and nondiabetic cataracts was decreased with an increase in the insoluble protein content. These results suggest that nonenzymatic glycosylation plays a role in the conformational change of lens proteins in both diabetic and nondiabetic cataracts.

AB - The free ε-amino groups and 5-hydroxymethylfurfural (5-HMF) contents were determined in soluble and insoluble proteins of clear human lenses and diabetic and nondiabetic senile cataractous lenses. The free ε-amino group content of soluble proteins in diabetic cataracts was decreased by 37% (P<0.01), whereas in nondiabetic senile cataracts it did not differ from that of clear lenses. The free ε-amino group content of insoluble proteins both in diabetic and nondiabetic cataracts was decreased significantly (P<0.001, P<0.015, respectively). The 5-HMF content of soluble proteins in diabetic cataracts was increased by 52% (P<0.001), whereas in nondiabetic cataracts it did not change from that of clear lenses. The 5-HMF content of insoluble proteins in diabetic as well as in nondiabetic cataracts was increased significantly as compared to that of clear lenses (P<0.001, P<0.001, respectively). The soluble protein of diabetic and nondiabetic cataracts was decreased with an increase in the insoluble protein content. These results suggest that nonenzymatic glycosylation plays a role in the conformational change of lens proteins in both diabetic and nondiabetic cataracts.

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