Free radical tissue damages in the anterior segment of the eye in experimental autoimmune uveitis

Sei Ichi Ishimoto, Guey Shuang Wu, Seiji Hayashi, Jie Zhang, Narsing A. Rao

Research output: Contribution to journalArticle

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Abstract

Purpose. To investigate increased free radical activity and the accumulation and localization of lipid peroxidation in the anterior segment of the eye with uveitis. Methods. Experimental autoimmune uveitis (EAU) was induced in rats using human S-antigen peptide. Conjugated dienes (CD) and keto-dienes (KD) were then extracted from the cornea, iris-ciliary body and lens of the EAU eyes. The quantity of CD and KD were determined by measuring ultraviolet absorption and estimating by means of a molar extinction coefficient. Frozen sections of EAU eyes were reacted with 3-hydroxy-2- naphthoic acid hydrazide (NAH), and NAH-carbonyl compounds were detected using a confocal laser scanning microscope. Statistical comparisons of CD and KD products between the EAU groups and controls were performed using the Student's t-test. Results. Compared to controls, CD and KD were significantly increased in the cornea and iris-ciliary body of EAU eyes. Lenses of EAU eyes showed a tendency to elevated levels of CD and KD. In EAU, primarily the anterior border layer and the posterior epithelium of the iris-and, to a lesser extent, the trabecular meshwork and corneal endothelium-revealed positive fluorescence staining for peroxidized carbonyl products. No staining was observed on the ciliary epithelium. Conclusions. Free radicals and lipid peroxidation products are generated in the anterior segment of the eye in EAU. Because the individual tissues in the anterior segment are composed of various levels of fatty acids and different concentrations of antioxidants, the extent of tissue damage from lipid peroxidation may represent a balance between the fatty acid composition and the antioxidant distribution in each of the tissues.

Original languageEnglish (US)
Pages (from-to)630-636
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number4
StatePublished - Mar 1996
Externally publishedYes

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Anterior Eye Segment
Uveitis
Free Radicals
Iris
Lipid Peroxidation
Ciliary Body
Cornea
Lenses
Fatty Acids
Epithelium
Antioxidants
Staining and Labeling
Trabecular Meshwork
Corneal Endothelium
Frozen Sections
Lasers
Fluorescence
Students
Antigens

All Science Journal Classification (ASJC) codes

  • Ophthalmology

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Free radical tissue damages in the anterior segment of the eye in experimental autoimmune uveitis. / Ishimoto, Sei Ichi; Wu, Guey Shuang; Hayashi, Seiji; Zhang, Jie; Rao, Narsing A.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 4, 03.1996, p. 630-636.

Research output: Contribution to journalArticle

Ishimoto, Sei Ichi ; Wu, Guey Shuang ; Hayashi, Seiji ; Zhang, Jie ; Rao, Narsing A. / Free radical tissue damages in the anterior segment of the eye in experimental autoimmune uveitis. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 4. pp. 630-636.
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