Function of phosphoenolpyruvate carboxykinase in mammary gland epithelial cells

Chang Wen Hsieh, Charles Huang, Ilya Bederman, Jianqi Yang, Michelle Beidelschies, Maria Hatzoglou, Michelle Puchowicz, Colleen M. Croniger

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Previously, we have shown that Pck1 expression in mammary gland adipocytes and white adipose tissue maintains triglyceride stores through glyceroneogenesis, and these lipids were used for synthesis of milk triglycerides during lactation. Reduced milk triglycerides during lactation resulted in patterning of the newborn for insulin resistance. In this study, the role of Pck1 in mammary gland epithelial cells was analyzed. The developmental expression of Pck1 decreased in isolated mouse mammary gland epithelial cells through development and during lactation. Using HC11, a clonal mammary epithelial cell line, we found that both Janus kinase 2 signal transducers and activators of transcription 5 and the AKT pathways contributed to the repression of Pck1 mRNA by prolactin. These pathways necessitate three accessory factor regions of the Pck1 promoter for repression by prolactin. Using [U- 13C6]glucose, [U-13C3] pyruvate, and [U-13C3]glycerol in HC11 cells, we determined that Pck1 functions in the pathway for the conversion of gluconeogenic precursors to glucose and contributes to glycerol-3-phosphate synthesis through glyceroneogenesis. Therefore, Pck1 plays an important role in both the mammary gland adipocytes and epithelial cells during lactation.

Original languageEnglish (US)
Pages (from-to)1352-1362
Number of pages11
JournalJournal of lipid research
Volume52
Issue number7
DOIs
StatePublished - Jul 1 2011

Fingerprint

Phosphoenolpyruvate
Human Mammary Glands
Lactation
Epithelial Cells
Triglycerides
Adipocytes
Prolactin
Milk
STAT5 Transcription Factor
Janus Kinase 2
Glucose
White Adipose Tissue
Accessories
Pyruvic Acid
Genetic Promoter Regions
Glycerol
Insulin Resistance
Breast
Insulin
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Hsieh, C. W., Huang, C., Bederman, I., Yang, J., Beidelschies, M., Hatzoglou, M., ... Croniger, C. M. (2011). Function of phosphoenolpyruvate carboxykinase in mammary gland epithelial cells. Journal of lipid research, 52(7), 1352-1362. https://doi.org/10.1194/jlr.M012666

Function of phosphoenolpyruvate carboxykinase in mammary gland epithelial cells. / Hsieh, Chang Wen; Huang, Charles; Bederman, Ilya; Yang, Jianqi; Beidelschies, Michelle; Hatzoglou, Maria; Puchowicz, Michelle; Croniger, Colleen M.

In: Journal of lipid research, Vol. 52, No. 7, 01.07.2011, p. 1352-1362.

Research output: Contribution to journalArticle

Hsieh, CW, Huang, C, Bederman, I, Yang, J, Beidelschies, M, Hatzoglou, M, Puchowicz, M & Croniger, CM 2011, 'Function of phosphoenolpyruvate carboxykinase in mammary gland epithelial cells', Journal of lipid research, vol. 52, no. 7, pp. 1352-1362. https://doi.org/10.1194/jlr.M012666
Hsieh CW, Huang C, Bederman I, Yang J, Beidelschies M, Hatzoglou M et al. Function of phosphoenolpyruvate carboxykinase in mammary gland epithelial cells. Journal of lipid research. 2011 Jul 1;52(7):1352-1362. https://doi.org/10.1194/jlr.M012666
Hsieh, Chang Wen ; Huang, Charles ; Bederman, Ilya ; Yang, Jianqi ; Beidelschies, Michelle ; Hatzoglou, Maria ; Puchowicz, Michelle ; Croniger, Colleen M. / Function of phosphoenolpyruvate carboxykinase in mammary gland epithelial cells. In: Journal of lipid research. 2011 ; Vol. 52, No. 7. pp. 1352-1362.
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